RT Journal Article
T1 Identification of new proteins related with cisplatin resistance in Saccharomyces cerevisiae
A1 Burgos-Molina, Antonio Manuel
A1 Mercado-Sáenz, Silvia
A1 Cárdenas, Casimiro
A1 López-Díaz, Beatriz
A1 Sendra-Portero, Francisco
A1 Ruiz-Gómez, Miguel José
K1 Saccharomyces cerevisiae
K1 Proteómica
AB The aim of this study is to select a cisplatin-resistant Saccharomyces cerevisiae strain to look for new molecular markers of resistance and the identification of mechanisms/interactions involved. A resistant strain was obtained after 80 days of cisplatin exposure. Then, total protein extraction, purification, and identification were carried out, in wild-type (wt) and resistant strains, by tandem mass spectrometry using a “nano HPLC-ESI-MS/MS” ion trap system. The increase in the exponentially modified protein abundance index (emPAI) (resistant vs wt strains) was calculated to study the increase in protein expression. “Genemania” software (http://www.Genemania.org/) was used to compare the effects, functions, and protein interactions. KEGG tool was used for metabolic pathway analysis. Data are available via ProteomeXchange with identifier PXD020665. The cisplatin-resistant strain showed 2.5 times more resistance than the wt strain for the inhibitory dose 50% (ID50) value (224 μg/ml vs 89.68 μg/ml) and 2.78 times more resistant for the inhibitory dose 90% (ID90) value (735.2 μg/ml vs 264.04 μg/ml). Multiple deregulated proteins were found in the glutathione and carbon metabolism, oxidative phosphorylation, proteasome, glycolysis and gluconeogenesis, glyoxylate metabolism, fatty acid degradation pathway, citric acid cycle, and ribosome. The most overexpressed proteins in the cisplatin-resistant strain were related to growth and metabolism (QCR2, QCR1, ALDH4, ATPB, ATPA, ATPG, and PCKA), cell structure (SCW10), and thermal shock (HSP26). The results suggest that these proteins could be involved in cisplatin resistance. The resistance acquisition process is complex and involves the activation of multiple mechanisms that interact together.
PB Springer
YR 2021
FD 2021
LK https://hdl.handle.net/10630/39499
UL https://hdl.handle.net/10630/39499
LA eng
NO Burgos-Molina AM, Mercado-Sáenz S, Cárdenas C, López-Díaz B, Sendra-Portero F, Ruiz-Gómez MJ. Identification of new proteins related with cisplatin resistance in Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2021;105(5):1965-1977. doi:10.1007/s00253-021-11137-w
NO Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI); Junta de Andalucía, code CTS-181.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026