RT Journal Article
T1 A novel eIF4E-interacting protein that forms non-canonical translation initiation complexes
A1 Toribio, René
A1 Muñoz, Alfonso
A1 Castro-Sanz, Ana B
A1 Merchante-Berg, Catharina
A1 Castellano, M Mar
K1 Proteínas vegetales
AB Translation is a fundamental step in gene expression that regulates multiple developmental and stress responses. One key step of translation initiation is the association between eIF4E and eIF4G. This process is regulated in different eukaryotes by proteins that bind to eIF4E; however, evidence of eIF4E-interacting proteins able to regulate translation is missing in plants. Here, we report the discovery of CERES, a plant eIF4E-interacting protein. CERES contains an LRR domain and a canonical eIF4E-binding site. Although the CERES–eIF4E complex does not include eIF4G, CERES forms part of cap-binding complexes, interacts with eIF4A, PABP and eIF3, and co-sediments with translation initiation complexes in vivo. Moreover, CERES promotes translation in vitro and general translation in vivo, while it modulates the translation of specific mRNAs related to light and carbohydrate response. These data suggest that CERES is a non-canonical translation initiation factor that modulates translation in plants.
PB Nature Research
YR 2019
FD 2019-12
LK https://hdl.handle.net/10630/29609
UL https://hdl.handle.net/10630/29609
LA eng
NO Toribio R, Muñoz A, Castro-Sanz AB, Merchante C, Castellano MM. A novel eIF4E-interacting protein that forms non-canonical translation initiation complexes. Nat Plants. 2019 Dec;5(12):1283-1296. doi: 10.1038/s41477-019-0553-2. Epub 2019 Dec 9. PMID: 31819221; PMCID: PMC6914366.
NO This research has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013) / ERC Grant Agreement n. 260468 to M.M.C. and from the grant S2013- ABI2748 from CAM. In addition, this work has been partially financial supported by RTI2018-095946-B100 from MICIU and by. "Severo Ochoa Programme for Centres of Excellence in R&D” from the Agencia Estatal de Investigación of Spain (grant SEV-2016-0672 (2017-2021) to the CBGP).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 25 ene 2026