RT Journal Article T1 Physiological, epigenetic and genetic regulation in some olive cultivars under salt stress. A1 Mousavi, Soraya A1 Regni, Luca A1 Bocchini, Marika A1 Mariotti, Roberto A1 Cultrera, Nicoló M. A1 Mancuso, Stefano A1 Googlani, Jalaladdin A1 Chakerolhosseini, Mohammad R. A1 Guerrero-Ruiz, Consolación María A1 Albertini, Emidio A1 Baldoni, Luciana A1 Proietti, Primo K1 Olivos - Genética K1 Plantas - Efectos de la sal K1 Plantas - Efectos del estrés AB Cultivated olive, a typical fruit crop species of the semi-arid regions, could successfully face the newscenarios driven by the climate change through the selection of tolerant varieties to salt and droughtstresses. In the present work, multidisciplinary approaches, including physiological, epigenetic andgenetic studies, have been applied to clarify the salt tolerance mechanisms in olive. Four varieties(Koroneiki, Royal de Cazorla, Arbequina and Picual) and a related form (O. europaea subsp. cuspidata)were grown in a hydroponic system under different salt concentrations from zero to 200 mM. In orderto verify the plant response under salt stress, photosynthesis, gas exchange and relative water contentwere measured at different time points, whereas chlorophyll and leaf concentration of Na+, K+ and Ca2+ions, were quantified at 43 and 60 days after treatment, when stress symptoms became prominent.Methylation sensitive amplification polymorphism (MSAP) technique was used to assess the effectsof salt stress on plant DNA methylation. Several fragments resulted differentially methylated amonggenotypes, treatments and time points. Real time quantitative PCR (RT-qPCR) analysis revealedsignificant expression changes related to plant response to salinity. Four genes (OePIP1.1, OePetD,OePI4Kg4 and OeXyla) were identified, as well as multiple retrotransposon elements usually targeted bymethylation under stress conditions. PB Springer Nature YR 2019 FD 2019-01-31 LK https://hdl.handle.net/10630/32479 UL https://hdl.handle.net/10630/32479 LA eng NO Mousavi, S., Regni, L., Bocchini, M. et al. Physiological, epigenetic and genetic regulation in some olive cultivars under salt stress. Sci Rep 9, 1093 (2019). https://doi.org/10.1038/s41598-018-37496-5 DS RIUMA. Repositorio Institucional de la Universidad de Málaga RD 19 ene 2026