RT Journal Article
T1 Effects of extracellular calcium ion on the germination of Gelidium floridanum (Rhodophyta, Gelidiales) tetraspores
A1 Bouvie, Fernanda
A1 Pereira, Débora Tomazi
A1 Ouriques, Luciane Cristina
A1 Costa Puerari, Rodrigo
A1 Matias, Willian Gerson
A1 Hayashi, Leila
A1 Simioni, Carmen
K1 Fisiología vegetal
K1 Esporas (Botánica)
K1 Rodofíceas
AB Gelidium species cultivation is rare because establishing one methodology is difficult, or even when this ispossible, the application to the field can be a challenge. These difficulties are associated with the low growthrates related to the availability of nutrients in culture media. The goal of this study was to observe the roleof extracellular calcium on spore germination. We investigated the effects of EGTA (a calcium-chelatingagent) and CaCl₂ (a source of extracellular calcium) in the germination of Gelidium floridanum tetraspores.Tetraspores were cultivated with EGTA and CaCl2 for 6 hours when the germ tube formation process wasinitiated. Adhesion, germination rate, and morphology analysis of the tetraspores were performed by lightmicroscopy; analysis of actin filaments and cell wall (cellulose) by confocal scanning laser microscopy;and ultrastructure analysis by transmission electron microscopy (TEM). There was no germination oftetraspores, and adhesion was reduced when treated with EGTA. A higher germination rate and accelerationof this process was observed when treated with CaCl2, indicating that germination and adhesion arecalcium-dependent. Tetraspores treated with EGTA show no cell wall formation or polarization of actinfilaments, confirmed by TEM, indicating interference with intracellular calcium homeostasis anddisorganization of organelles. These results contrasted with those observed in CaCl2 treatment, where asmall amount of extracellular calcium accelerates the germinative process. Based on these results, weconclude that this species has the germination dependent on extracellular Ca2+ influx, and its suppressionmay cause the interruption of Ca2+-dependent signaling required for tetraspores germination.
PB Elsevier
YR 2025
FD 2025
LK https://hdl.handle.net/10630/40472
UL https://hdl.handle.net/10630/40472
LA eng
NO Fernanda Bouvie, Débora Tomazi Pereira, Luciane Cristina Ouriques, Rodrigo Costa Puerari, Willian Gerson Matias, Leila Hayashi and Carmen Simioni, Effects of extracellular calcium ion on the germination of Gelidium floridanum (Rhodophyta, Gelidiales) tetraspores, Micron, (2025) doi:https://doi.org/10.1016/j.micron.2025.103942
NO Funding for open access charge: Universidad de Málaga/CBUA
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 22 ene 2026