RT Journal Article
T1 Isolation of Mycosporine-like Amino Acids from Red Macroalgae and a Marine Lichen by High-Performance Countercurrent Chromatography: A Strategy to Obtain Biological UV-Filters.
A1 Vega Sánchez, Julia
A1 Bárcenas-Pérez, Daniela
A1 Fuentes-Ríos, David
A1 López-Romero, Juan Manuel
A1 Hrouzek, Pavel
A1 López-Figueroa, Félix
A1 Cheel, José
K1 Aminoácidos
K1 Líquenes
K1 Cromatografía
K1 Algas marinas
K1 Biotecnología marina
AB Marine organisms have gained considerable biotechnological interest in recent years due to their wide variety of bioactive compounds with potential applications. Mycosporine-like amino acids (MAAs) are UV-absorbing secondary metabolites with antioxidant and photoprotective capacity, mainly found in organisms living under stress conditions (e.g., cyanobacteria, red algae, or lichens). In this work, five MAAs were isolated from two red macroalgae (Pyropia columbina and Gelidium corneum) and one marine lichen (Lichina pygmaea) by high-performance countercurrent chromatography (HPCCC). The selected biphasic solvent system consisted of ethanol, acetonitrile, saturated ammonium sulphate solution, and water (1:1:0.5:1; v:v:v:v). The HPCCC process for P. columbina and G. corneum consisted of eight separation cycles (1 g and 200 mg of extract per cycle, respectively), whereas three cycles were performed for of L. pygmaea (1.2 g extract per cycle). The separation process resulted in fractions enriched with palythine (2.3 mg), asterina-330 (3.3 mg), shinorine (14.8 mg), porphyra-334 (203.5 mg) and mycosporine-serinol (46.6 mg), which were subsequently desalted by using precipitation with methanol and permeation on a Sephadex G-10 column. Target molecules were identified by HPLC, MS, and NMR.
PB MDPI
YR 2023
FD 2023-06-10
LK https://hdl.handle.net/10630/27133
UL https://hdl.handle.net/10630/27133
LA eng
NO Vega J, Bárcenas-Pérez D, Fuentes-Ríos D, López-Romero JM, Hrouzek P, Figueroa FL, Cheel J. Isolation of Mycosporine-like Amino Acids from Red Macroalgae and a Marine Lichen by High-Performance Countercurrent Chromatography: A Strategy to Obtain Biological UV-Filters. Marine Drugs. 2023; 21(6):357. https://doi.org/10.3390/md21060357
NO Partial funding for open access charge: Universidad de Málaga
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026