RT Journal Article
T1 High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci.
A1 Mérida García, Rosa
A1 Gálvez-Rojas, Sergio
A1 Paux, Etienne
A1 Dorado-Pérez, Gabriel
A1 Pascual, Laura
A1 Giraldo, Patricia
A1 Hernández Molina, Pilar
K1 Trigo - Aspectos moleculares
AB The practical use of molecular markers is facilitated by cost-effective detection techniques. In this work, wheat insertion site-based polymorphisms (ISBP) markers were set up for genotyping using high-resolution melting analysis (HRM). Polymorphic HRM-ISBP assays were developed for wheat chromosomes 4A and 3B and used for wheat variability assessment. The marker sequences were mapped against the wheat genome reference sequence, targeting interesting genes. Those genes were located within or in proximity to previously described quantitative trait loci (QTL) or meta-quantitative trait loci (MQTL) for drought and heat stress tolerance, and also yield and yield related traits. Eighteen of the markers used tagged drought related genes and, interestingly, eight of the genes were differentially expressed under different abiotic stress conditions. These results confirmed HRM as a cost-effective and efficient tool for wheat breeding programs.
PB MDPI
YR 2020
FD 2020-09-01
LK https://hdl.handle.net/10630/36172
UL https://hdl.handle.net/10630/36172
LA eng
NO This research was funded by project P18-RT-992 from Junta de Andalucía (Andalusian Regional Government), Spain (Co-funded by FEDER), and by the Spanish Ministry of Science and Innovation project PID2019-109089RB-C32.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026