RT Conference Proceedings
T1 Intregrated transcriptome, methylome and smallRNA profile analysis of a geminivirus infection
A1 Piedra-Aguilera, Álvaro
A1 Jiao, Chen
A1 Esteve-Codina, Anna
A1 Abad, Marc
A1 Pérez-Luna, Ana Isabel
A1 Villanueva-Montiel, Francisco
A1 Díaz-Pendón, Juan Antonio
A1 Fei, Zhangjun
A1 Rodríguez-Bejarano, Eduardo
A1 Castillo-Garriga, Araceli
K1 Virus fitopatógenos
AB Geminiviruses constitute the largest family of plant-infecting viruses with small, single- stranded DNA genomes that replicate through double-stranded DNA intermediates. Because of their limited coding capacity, geminiviruses use plant nuclear machiney to amplify their genomes, which are packaged into nucleosomes forming chromatin as multiple circular minichromosomes. Thus, viral minichromosomes must encounter the nuclear pathways that regulate host gene expression and chromatin states. DNA methylation and post-transcriptional gene silencing play critical roles in controlling infection of geminiviruses and this pathogen can counteract these host defense mechanisms and promote its infectivity.To better understand this virus-host interaction at a genetic and epigenetic level we have analysed the transcriptome, sRNA profile and methylome of tomato plants infected with the geminivirus, TYLCV (Tomato yellow leaf curl virus). Total RNA and DNA was extracted from tomato–infected plants (three biological replicates) and analysed at 2, 7, 14 and 21 days pots-infection (dpi). Analysis of the changes in host transcription during the infection and its correlation with changes in sRNA profiles and DNA methylation will be discussed/shown.This work represents the first comprehensive study of the genetic and epigenetics interactions established during a geminivirus infection in its natural host.
YR 2018
FD 2018-01-08
LK https://hdl.handle.net/10630/14985
UL https://hdl.handle.net/10630/14985
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026