RT Conference Proceedings
T1 Arginine biosynthesis and utilization in maritime pine
A1 Llebrés, María Teresa
A1 Pascual-Moreno, María Belén
A1 Debille, Sandrine
A1 Trontin, JF
A1 Harvengt, Luc
A1 Ávila-Sáez, Concepción
A1 Cánovas-Ramos, Francisco Miguel
K1 Biología molecular vegetal
AB Vegetative propagation through somatic embryogenesis in combination with the cryopreservationof embryogenic lines is a major tool in conifer biotechnology. An important process during thematuration phase of embryogenesis is the biosynthesis and deposition of storage proteins. Theaccumulation of some abundant storage proteins in maturing cotyledonary somatic embryos (SE)is much lower than in mature zygotic embryos (ZE) showing an important influence of storagecompounds on the quality of SE. Arginine constitutes a large portion of the amino acid pool instorage proteins of conifers and therefore arginine biosynthesis and utiization is a relevantmetabolic pathway during pine embryogenesis and early growth.Research in our laboratory is focused on maritime pine (Pinus pinaster Ait.), a broadly plantedconifer species in France, Spain and Portugal where it is distributed over approximately 4 millionhectares. This conifer species is also one of the most advanced model trees for genetic andphenotypic studies and a large number of molecular and transcriptomic resources are currentlyavailable.With the aim to understand the molecular basis of the differential accumulation of storage proteinsin SE and ZE, the arginine metabolic pathway has been studied in maritime pine, in collaborationwith the French private institute FCBA. A general overview of this research programme will bepresented and discussed. The knowledge acquired from our studies will help to refinebiotechnological procedures for clonal propagation of conifers via somatic embryogenesis.Funding support by:The Spanish Ministerio de Economía y Competitividad (BIO2015-69285-R)and Junta de Andalucía (BIO-474). And the French Ministry of Agriculture (DGAL, N°2014-352,QuaSeGraine project). The project also benefited from the technical support of theXYLOBIOTECH facility (ANR-10-EQPX-16 XYLOFOREST).
YR 2017
FD 2017-10-30
LK http://hdl.handle.net/10630/14736
UL http://hdl.handle.net/10630/14736
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026