RT Journal Article T1 Co-occurrence of viral and bacterial pathogens in disease outbreaks affecting newly cultured sparid fish A1 García-Rosado, Esther A1 Cano, Irene A1 Martín-Antonio, Beatriz A1 Labella Vera, Alejandro Manuel A1 Manchado, Manuel A1 Alonso, María del Carmen A1 Castro, Dolores A1 Borrego, Juan José K1 Espáridos - Enfermedades K1 Espáridos - Virus K1 Bacterias patógenas AB Several microbial disease outbreaks in farm stocks of newly cultured sparid fish species, such as commonseabream, redbanded seabream, and white seabream, were recorded from 2004 to 2006. This study describes the isolation andcharacterization of the potential causative agents, either bacteria or viruses, of these outbreaks. The isolated bacterial strainswere characterized according to traditional taxonomical analyses and sequencing of a 16S rDNA fragment. Most bacteriawere identified as Vibrio spp. and Photobacterium damselae subsp. damselae. The development of cytopathic effects (CPE)on different fish cell lines, the application of specific nested-PCR tests for infectious pancreatic necrosis virus (IPNV), viralnervous necrosis virus (VNNV) and viral hemorrhagic septicemia virus (VHSV), and subsequent sequence analyses wereused for virus detection and identification. VNNV, related to the striped jack neural necrosis virus (SJNNV) genotype, andVHSV, related to the genotype Ia, were the only viruses detected. VNNV was isolated from the three fish species under studyin five different outbreaks, whereas VHSV was isolated from common seabream and white seabream during two of these outbreaks.IPNV was not detected in any case. PB Springer YR 2007 FD 2007-09-01 LK https://hdl.handle.net/10630/33991 UL https://hdl.handle.net/10630/33991 LA eng NO García-Rosado E, Cano I, Martín-Antonio B, Labella A, Manchado M, Alonso MC, Castro D, Borrego JJ. Co-occurrence of viral and bacterial pathogens in disease outbreaks affecting newly cultured sparid fish. Int Microbiol. 2007 Sep;10(3):193-9. PMID: doi: 18076001.10.2436/20.1501.01.27 DS RIUMA. Repositorio Institucional de la Universidad de Málaga RD 20 ene 2026