RT Journal Article
T1 Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress
A1 Ruiz-López, Noemí
A1 Pérez-Sancho, Jessica
A1 Esteban del Valle, Alicia
A1 Haslam, Richard P.
A1 Vanneste, Steffen
A1 Catalá, Rafael
A1 Perea-Resa, Carlos
A1 Van Damme, Daniël
A1 García-Hernández, Selene
A1 Albert, Armando
A1 Vallarino, José
A1 Lin, Jinxing
A1 Friml, Jiří
A1 Macho, Alberto P
A1 Salinas, Julio
A1 Rosado, Abel
A1 Napier, Johnathan A.
A1 Amorim-Silva, Vitor
A1 Botella-Mesa, Miguel Ángel
K1 Membrana celular
K1 Proteínas de membranas
K1 Retículo endoplásmico
AB Endoplasmic reticulum–plasma membrane contact sites (ER–PM CS) play fundamental roles in all eukaryotic cells. Arabidopsis thaliana mutants lacking the ER–PM protein tether synaptotagmin1 (SYT1) exhibit decreased PM integrity under multiple abiotic stresses, such as freezing, high salt, osmotic stress, and mechanical damage. Here, we show that, together with SYT1, the stress-induced SYT3 is an ER–PM tether that also functions in maintaining PM integrity. The ER–PM CS localization of SYT1 and SYT3 is dependent on PM phosphatidylinositol-4-phosphate and is regulated by abiotic stress. Lipidomic analysis revealed that cold stress increased the accumulation of diacylglycerol at the PM in a syt1/3 double mutant relative to wild-type while the levels of most glycerolipid species remain unchanged. In addition, the SYT1-green fluorescent protein fusion preferentially binds diacylglycerol in vivo with little affinity for polar glycerolipids. Our work uncovers a SYT-dependent mechanism of stress adaptation counteracting the detrimental accumulation of diacylglycerol at the PM produced during episodes of abiotic stress.
PB Oxford University Press in behalf of American Society of Plant Biologists
YR 2021
FD 2021-05-04
LK https://hdl.handle.net/10630/34509
UL https://hdl.handle.net/10630/34509
LA eng
NO Noemi Ruiz-Lopez, Jessica Pérez-Sancho, Alicia Esteban del Valle, Richard P. Haslam, Steffen Vanneste, Rafael Catalá, Carlos Perea-Resa, Daniël Van Damme, Selene García-Hernández, Armando Albert, José Vallarino, Jinxing Lin, Jiří Friml, Alberto P. Macho, Julio Salinas, Abel Rosado, Johnathan A. Napier, Vitor Amorim-Silva, Miguel A. Botella, Synaptotagmins at the endoplasmic reticulum–plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress, The Plant Cell, Volume 33, Issue 7, July 2021, Pages 2431–2453, https://doi.org/10.1093/plcell/koab122
NO This work was supported by the Ministerio de Economía y Competitividad, co-financed by the European Regional Development Fund (grant no. BIO2017-82609-R to M.A.B.), the Ministerio de Ciencia, Innovación y Universidades (grant no. PGC2018-098789-B-I00 to N.R.-L.) UMA-FEDER (grant UMA18-FEDERJA-154 to N.R.-L.), and the Marie Skłodowska-Curie actions (grant no. H2020-655366-IIF- PLICO to M.A.B. and N.R.-L.). N.R.L. was supported by the Ramón y Cajal program RYC-2013-12699 (MINECO, Spain). J.P.-S. and S.G.-H. were funded by the Ministerio de Economía y Competitividad in Formación del Personal Investigador Fellowship (grant no. BES-2012-052324) and (PRE2018-085284), respectively. R.P.H. and J.A.N. received support from the Biotechnology and Biological Sciences Research Council (BBSRC, UK) in the form of an Institute Strategic Programme Grant (grant no. BBS/E/C/000I0420). J.L. is supported by the Program of Introducing Talents of Discipline to Universities (111 Project, grant no. B13007). A.P.M. and J.P.-S. were supported by the Shanghai Center for Plant Stress Biology (Chinese Academy of Sciences), Chinese 1000 Talents Program. A.R. was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC-Discovery Grant no. RGPIN-2019-05568). Support was also provided by AEI/FEDER, UE (grant nos. BIO2016-79187-R and PID2019-106987RB-I00 to J.P.-S.) and by the European Research Council under the European Union's Seventh Framework Programme (grant no. FP7/2007-2013)/ERC grant agreement no. 742985 to J.F. and T-Rex (project number 682436 to D.V.D.).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026