RT Journal Article
T1 Hepatic and serum branched-chain fatty acid profile in patients with nonalcoholic fatty liver disease: A case–control study
A1 Martínez-Montoro, José Ignacio
A1 Nuñez-Sanchez, María Ángeles
A1 Martinez-Sanchez, María Antonia
A1 Balaguer-Román, Andrés
A1 Fernández-Ruiz, Virginia E.
A1 Ferrer-Gómez, Mercedes
A1 Sledzinski, Tomasz
A1 Frutos, María Dolores
A1 Fernández-García, José Carlos
A1 Mika, Adriana
A1 Ramos-Molina, Bruno
K1 Hígado -- Enfermedades
K1 Fisiología patológica
AB ObjectiveAlterations in the hepatic lipidome are a crucial factor involved in the pathophysiology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to evaluate the serum and hepatic profile of branched-chain fatty acids (BCFAs) in patients with different stages of NAFLD.MethodsThis was a case–control study performed in 27 patients without NAFLD, 49 patients with nonalcoholic fatty liver, and 17 patients with nonalcoholic steatohepatitis, defined by liver biopsies. Serum and hepatic levels of BCFAs were analyzed by gas chromatography–mass spectrometry. The hepatic expression of genes involved in the endogenous synthesis of BCFAs was analyzed by real-time quantitative polymerase chain reaction (RT-qPCR).ResultsA significant increase in hepatic BCFAs was found in subjects with NAFLD compared with those without NAFLD; no differences were observed in serum BCFAs between study groups. Trimethyl BCFAs, iso-BCFAs, and anteiso-BCFAs were increased in subjects with NAFLD (either nonalcoholic fatty liver or nonalcoholic steatohepatitis) compared with those without NAFLD. Correlation analysis showed a relationship between hepatic BCFAs and the histopathological diagnosis of NAFLD, as well as other histological and biochemical parameters related to this disease. Gene expression analysis in liver showed that the mRNA levels of BCAT1, BCAT2, and BCKDHA were upregulated in patients with NAFLD.ConclusionsThese results suggest that the increased production of liver BCFAs might be related to NAFLD development and progression.
PB Wiley
YR 2023
FD 2023
LK https://hdl.handle.net/10630/26381
UL https://hdl.handle.net/10630/26381
LA eng
NO Martínez‐Montoro, Núñez‐Sánchez, M. Á., Martinez‐Sanchez, M. A., Balaguer‐Román, A., Fernández‐Ruiz, V. E., Ferrer‐Gómez, M., Sledzinski, T., Frutos, M. D., Fernández‐García, J. C., Mika, A., & Ramos‐Molina, B. (2023). Hepatic and serum branched‐chain fatty acid profile in patients with nonalcoholic fatty liver disease: A case–control study. Obesity (Silver Spring, Md.), 31(4), 1064–1074. https://doi.org/10.1002/oby.23711
NO This work was funded by the Institute of Health “Carlos III” (ISCIII) and cofunded by the Fondo Europeo de Desarrollo Regional-FEDER (grant number PI20/00505). J.C.F-G was supported by an intensification research program (INT21/00078, ISCIII, Spain; cofunded by the Fondo Europeo de Desarrollo Regional-FEDER), M.A.M-S was supported by a PFIS predoctoral fellowship from the ISCIII (FI21/00003, ISCIII, Spain; cofunded by the Fondo Europeo de Desarrollo Regional-FEDER), and B.R-M was supported by the “Miguel Servet Type I” program (CP19/00098, ISCIII, Spain; cofunded by the Fondo Europeo de Desarrollo Regional-FEDER). The funding organizations played no role in the design of the study, review and interpretation of the data, or final approval of the manuscript. Funding for open access charge: Universidad de Málaga / CBUA
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026