RT Conference Proceedings
T1 Localization and characterization of SMP-containing proteins in Membrane Contact Sites
A1 Huércano Rubens, Carolina
A1 Sánchez-Vera, Victoria
A1 Percio-Vargas, Francisco
A1 Morello-López, Jorge
A1 Botella-Mesa, Miguel Ángel
A1 Ruiz-López, Noemí
K1 Proteína SMP
K1 Lípidos
K1 Biología vegetal - Congresos
AB Membrane contact sites (MCS) are discrete regions where two membranes from different organelles are closely apposed(10-30 nm). In those regions, non-vesicular transfer of lipids takes place to ensure proper organelle functioning. Arabidopsis SYT1 is one of the best characterized MCS protein, and it plays a relevant role in tolerance to abiotic stresses.SYT1 is a SMP (synaptotagmin-like mitocondrial lipid binding domain) containing protein localized at ER-PM contactsites. Recent studies suggest that this protein transfer glycerolipids between these two membranes. However, little isknown about other SMP-containing proteins in plants, as their localization or their role in abiotic stress.We have focused on studying the rest of SMP-containing proteins in Arabidopsis thaliana and Solanum lycopersicum. Toidentify them, human E-Syt1 sequence was used to find the remote orthologues in plants. An interesting highlight of thoseresults was that some SMP-containing proteins are exclusive from plants, there are no orthologues in human nor yeast. Thesubsequent step was the study of their subcellular location, that was carried out in Nicotiana benthamiana by transientexpression of the SMP-containing proteins from Arabidopsis and Solanum, followed by confocal microscopy imaging. Wehave found that those proteins locate in different MCS across the cell: SYT6, NTMC2T6 and Tex2 localise in ER-Golgicontact sites, NTMC2T5 in ER-Chloroplast contact sites, and we have also confirmed that Solanum CLB1 and SYT5localized at ER-PM contact sites as their Arabidopsis counterparts. Additionally, we have analysed the root growth, seedgermination rates and fully expanded cotyledons of Arabidopsis mutants for these genes in media supplemented with saltor ABA, and our results suggest that some of these proteins might be implicated in abiotic stress signalling through anABA pathway.
YR 2021
FD 2021
LK https://hdl.handle.net/10630/22764
UL https://hdl.handle.net/10630/22764
LA spa
NO This work is supported by grants from: Ministerio de Ciencia, Innovación y Universidades (grant PGC2018-098789-B-I00), UMA-FEDER (grant UMA18-FEDERJA-154) and Ministerio de Ciencia e Innovación (BIO2017-82609-R), and meeting assistance was granted by Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026