RT Journal Article
T1 Properties and functional análisis of two chorismate mutases from maritime pine.
A1 De-la-Torre-Fazio, Fernando Nicolás
A1 Medina Morales, Beatriz
A1 Blanca Reyes, Irene Victoria
A1 Pascual-Moreno, María Belén
A1 Ávila-Sáez, Concepción
A1 Cánovas-Ramos, Francisco Miguel
A1 Castro-Rodríguez, Vanessa Viviana
K1 Genética forestal
K1 Pino marítimo
AB Through the shikimate pathway, a massive metabolic flux connects the central carbon metabolism with the synthesis of chorismate, the common precursor of the aromatic amino acids phenylalanine, tyrosine, and tryptophan, as well as other compounds, including salicylate or folate. The alternative metabolic channeling of chorismate involves a key branch-point, finely regulated by aromatic amino acid levels. Chorismate mutase catalyzes the conversion of chorismate to prephenate, a precursor of phenylalanine and tyrosine and thus a vast repertoire of fundamental derived compounds, such as flavonoids or lignin. The regulation of this enzyme has been addressed in several plant species, but no study has included conifers or other gymnosperms, despite the importance of the phenolic metabolism for these plants in processes such as lignification and wood formation. Here, we show that maritime pine (Pinus pinaster Aiton) has two genes that encode for chorismate mutase, PpCM1 and PpCM2. Our investigations reveal that these genes encode plastidial isoenzymes displaying activities enhanced by tryptophan and repressed by phenylalanine and tyrosine. Using phylogenetic studies, we have provided new insights into the possible evolutionary origin of the cytosolic chorismate mutases in angiosperms involved in the synthesis of phenylalanine outside the plastid. Studies based on different platforms of gene expression and co-expression analysis have allowed us to propose that PpCM2 plays a central role in the phenylalanine synthesis pathway associated with lignification
PB MDPI
YR 2024
FD 2024
LK https://hdl.handle.net/10630/37943
UL https://hdl.handle.net/10630/37943
LA eng
NO : This work was supported by grants from Ministry of Science and Innovation (PID2021-125040OB-I00, RTI2018-094041-B-I00 and PID2021-128168NB-100) and Junta de Andalucía (researchgroup BIO-114, VCR PAIDI contract DOC_00167). I.B.-R. was supported by a grant from the SpanishMinistry of Education (FPI) and B.M.-M. was supported via PID2021-125040OB-I00 funding
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026