RT Journal Article
T1 Study of peritoneal macrophage immunophenotype in sheep experimentally infected with Fasciola hepatica
A1 Ruiz-Campillo, María Teresa
A1 Molina-Hernández, Verónica
A1 Pérez, José
A1 Pacheco Luque, Isabel
A1 Pérez-Caballero, Raúl
A1 Escamilla-Sánchez, Alejandro
A1 Martínez-Moreno, Francisco Javier
A1 Martinez Moreno, Álvaro
A1 Zafra, Rafael
K1 Inmunopatología
AB During Fasciola hepatica infection, the parasite has the capability to modulate the host immune response towardsa non-protector Th2 type instead of Th1. This type of immune response is closely related to the alternativeactivation of macrophages (M2 profile) as has been shown in vivo in murine models. In this study, an experimentwas carried out in order to evaluate the expression of CD68, CD14, CD206 and iNOS in cells present in theperitoneal fluid of sheep during early stages of infection with F. hepatica (1, 3, 9 and 18 days post-infection, dpi)by immunocytochemistry. To the authors’ knowledge, this is the first report that studies the in vivo immunophenotype of macrophages from the peritoneal fluid of sheep infected with F. hepatica. Throughout theexperiments the absolute number of leucocytes progressively increased, reaching its highest value at 18 dpi,mainly due to the increase of eosinophils. This immunocytochemical study had two purposes: 1) CD68 expression was assessed with Hansel counterstaining, to optimally identify peritoneal macrophages, eosinophilsand lymphocytes; 2) expression of CD14, CD206 and iNOS was evaluated to identify alternative or classicalpathways of macrophage activation. The results showed a significant increase in CD14 from day 3 dpi comparedwith the non-infected group. CD206 expression at all time-points showed a significant and dramatic increase incomparison with the uninfected group. On the other hand, iNOS expression showed little variation, and wassignificantly decreased at 18 dpi in comparison with the uninfected group. These results suggest that F. hepaticainduces an alternative activation of peritoneal macrophages of sheep from the first day post-infection, whichmay facilitate parasite survival. This is the first report describing M2 activation of peritoneal macrophages inruminants infected with F. hepatica.
PB Elsevier B.V.
YR 2018
FD 2018-06-15
LK https://hdl.handle.net/10630/29921
UL https://hdl.handle.net/10630/29921
LA eng
NO M.T. Ruiz-Campillo, V. Molina-Hernández, J. Pérez, I.L. Pacheco, R. Pérez, A. Escamilla, F.J. Martínez-Moreno, A. Martínez-Moreno, R. Zafra, Study of peritoneal macrophage immunophenotype in sheep experimentally infected with Fasciola hepatica, Veterinary Parasitology, Volume 257, 2018, Pages 34-39, ISSN 0304-4017, https://doi.org/10.1016/j.vetpar.2018.05.019. (https://www.sciencedirect.com/science/article/pii/S0304401718302127)
NO Financiación y miembro investigador en proyectos europeos H2020-SFS-2014-2-635408 PARAGONE - Vaccines for animal parasites” y un Proyecto Nacional INTERFAS (AGL2015-67023-C2-1-R9)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026