RT Journal Article
T1 Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica
A1 Ruiz-Campillo, María Teresa
A1 Molina-Hernández, Verónica
A1 Escamilla-Sánchez, Alejandro
A1 Stevenson, Michael
A1 Pérez, José
A1 Martinez Moreno, Álvaro
A1 Donnelly, Sheila
A1 Dalton, John P.
A1 Cwiklinski, Krystyna
K1 Inmunopatología
AB Immune signatures of sheep acutely-infected with Fasciola hepatica, an important pathogen of livestock and humans were analysed within the peritoneal compartment to investigate early infection. Within the peritoneum, F. hepatica antibodies coincided with an intense innate and adaptive cellular immune response, with infiltrating leukocytes and a marked eosinophilia (49%). However, while cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFβ were elevated, these were not statistically different at 18 days post-infection compared to uninfected animals indicating that the immune response is muted and not yet skewed to a Th2 type response that is associated with chronic disease. Proteomic analysis of the peritoneal fluid identified infection-related proteins, including several structural proteins derived from the liver extracellular matrix, connective tissue and epithelium, and proteins related to the immune system. Periostin and vascular cell adhesion protein 1 (VCAM-1), molecules that mediate leukocyte infiltration and are associated with inflammatory disorders involving marked eosinophilia (e.g. asthma), were particularly elevated in the peritoneum. Immuno-histochemical studies indicated that the source of periostin and VCAM-1 was the inflamed sheep liver tissue. This study has revealed previously unknown aspects of the immunology and pathogenesis associated with acute fascioliasis in the peritoneum and liver.
PB Springer Nature
YR 2017
FD 2017-06-05
LK https://hdl.handle.net/10630/29092
UL https://hdl.handle.net/10630/29092
LA eng
NO Ruiz-Campillo, M. T., Molina Hernandez, V., Escamilla, A., Stevenson, M., Perez, J., Martinez-Moreno, A., Donnelly, S., Dalton, J. P., & Cwiklinski, K. (2017). Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica. Scientific reports, 7(1), 2782.
NO European Research Council Advanced Grant (HELIVAC, 322725) Horizon 2020-funded Consortium PARAGONE
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026