RT Conference Proceedings
T1 Transcriptomic and proteomic approaches to study bacterial wilt
T2 Aproximaciones transcriptómicas y proteómicas al estudio de la marchitez bacteriana
A1 Valls, Marc
K1 Plantas - Enfermedades bacterianas
AB Ralstonia solanacearum is a ß-proteobacterium that causes bacterial wilt on important crops such as tomato and potato. We applied two complementary approaches to study R. solanacearum virulence determinants.First, we carried out transcriptomic to define the genetic programme deployed by the bacterium to infect plants. We detected expression for >90% R. solanacearum genes both from isolated bacteria or in silico selected transcripts sequenced from infected plant tissues. Global transcriptional profiling provided insight into the intercellular environment encountered by this plant pathogen and the carbon and energy sources it utilizes during plant infection. In addition, we identified several R. solanacearum genes that are significantly up-regulated during infection but had not been previously identified as virulence factors. Second, we applied of activity-based protein profiling to dissect changes in protein activity changes that occur in tolerant or susceptible tomato plants in response to R. solanacearum. Our work reveals specific activation of papain-like cysteine proteases and serine hydrolases in the leaf apoplast of the tolerant tomato Hawaii 7996 upon R. solanacearum infection. In addition, protein network analysis showed that deeper changes in network topology take place in the susceptible tomato variety,suggesting that the tolerant cultivar might be more prepared to face R. solanacearum in its basal state. Our work will help identifying bacterial virulence genes key for successful plant infection, which can be targets for novel antibacterial drugs, and plant response activities that can assist in breeding for disease resistance.
YR 2018
FD 2018-07-09
LK https://hdl.handle.net/10630/16161
UL https://hdl.handle.net/10630/16161
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 24 ene 2026