RT Journal Article
T1 Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains.
A1 Rufián Plaza, José Sebastián
A1 Macho, Alberto P
A1 Corry, David S
A1 Mansfield, John W
A1 Ruiz-Albert, Francisco Javier
A1 Arnold, Dawn L.
A1 Beuzón-López, Carmen del Rosario
K1 Pseudomonas syringae - Genética
K1 Virulencia (Microbiología)
K1 Bacterias fitopatógenas
AB Recent advances in genomics and single-cell analysis have demonstrated the extraordinary complexity reached by microbial populations within their hosts. Communities range from complex multispecies groups to homogeneous populations differentiating into lineages through genetic or non-genetic mechanisms. Diversity within bacterial populations is recognized as a key driver of the evolution of animal pathogens. In plants, however, little is known about how interactions between different pathogenic and non-pathogenic variants within the host impact on defence responses, or how the presence within a mixture may affect the development or the fate of each variant. Using confocal fluorescence microscopy, we analysed the colonization of the plant apoplast by individual virulence variants of Pseudomonas syringae within mixed populations. We found that non-pathogenic variants can proliferate and even spread beyond the inoculated area to neighbouring tissues when in close proximity to pathogenic bacteria. The high bacterial concentrations reached at natural entry points promote such interactions during the infection process. We also found that a diversity of interactions take place at a cellular level between virulent and avirulent variants, ranging from dominant negative effects on proliferation of virulent bacteria to in trans suppression of defences triggered by avirulent bacteria. Our results illustrate the spatial dynamics and complexity of the interactions found within mixed infections, and their potential impact on pathogen evolution.
PB Wiley
YR 2018
FD 2018
LK https://hdl.handle.net/10630/32488
UL https://hdl.handle.net/10630/32488
LA eng
NO Rufián JS, Macho AP, Corry DS, Mansfield JW, Ruiz-Albert J, Arnold DL, Beuzón CR. Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains. Mol Plant Pathol. 2018 Mar;19(3):537-551. doi: 10.1111/mpp.12539. Epub 2017 Apr 11.
NO Política de acceso abierto tomada de:  https://bsppjournals.onlinelibrary.wiley.com/hub/journal/13643703/homepage/article_publication_charges.html
NO Ministerio de Economía y Competitividad . Grant Numbers: BIO2015-64391-R , BIO2012-35641Ministerio de Ciencia e Innovación . Grant Numbers: AGL2010-22287-C02-2 , BIO2012-35641
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 24 ene 2026