RT Conference Proceedings T1 New indolium fluorofores for two-photon absortion (2PA) bioimaging applications A1 Pérez-de-Inestrosa-Villatoro, Ezequiel A1 Benítez-Martín, Carlos A1 Nájera-Albendín, Francisco K1 Fotoquímica K1 Fotobioquímica AB The development of organic materials with 2PA has attracted intensive attention in the past two decades [1]. In two-photon bio-imaging applications the design of the chromophore requires to have a good cross-section (σ2PA) and good biological compatibility which depends on the molecular volume and polarity [2].In this work, we present the design, synthesis and characterization of new indoliumderivatives. These compounds are easy to achieve with good yields and good photophysicalproperties. In addition, time-dependent density functional theory (TDDFT) has been carried out toinvestigate the energy level of the ground and excited state.Their spectral properties and assays performed on cultured cells, demonstrate the potentialof these compounds as fluorescent probes with application in two-photon bio-imaging. YR 2016 FD 2016 LK http://hdl.handle.net/10630/11990 UL http://hdl.handle.net/10630/11990 LA eng NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Ministerio de Ciencia e Innovación-Spain (CTQ2013-41339-P and CTQ2015-71896-REDT) DS RIUMA. Repositorio Institucional de la Universidad de Málaga RD 20 ene 2026