RT Conference Proceedings
T1 Diversity of plaque-associated myeloid cells subtypes in human alzheimer’s disease brain
A1 Mejías-Ortega, Marina
A1 Sánchez-Mejías, Elisabeth
A1 Muñoz-Castro, Clara
A1 Trujillo-Estrada, Laura Isabel
A1 Vizuete, María Luisa
A1 Vitorica Ferrández, Javier
A1 Gutiérrez-Pérez, Antonia
K1 Sistema nervioso - Degeneración
K1 Alzheimer, Enfermedad de
AB Aims: Parenchymal microglia, as well other myeloid cells, have been postulated as a critical factor in Alzheimer´s disease (AD) pathogenesis since the identification of genetic risk factors related to their functions. However, the different phenotypes and the implication of the diverse immune cells in the human pathology have not been determined yet. In this work, we have further analyzed the phenotypic profile of the damage-associated myeloid cells in two AD vulnerable brain regions, the frontal cortex and hippocampus. Methods: Immunohistochemistry and image analysis approaches have been carried out in postmortem brain samples from patients with AD (Braak V-VI) and aged controls without neurological symptoms (Braak II). Results: Damage-associated microglial cells were clustered around amyloid plaques and expressed Iba1, TMEM119, CD68, Trem2 and CD45high. Moreover, AD brains exhibited parenchymal infiltration of CD163-positive monocyte-derived cells that invaded plaque near blood vessels. While the frontal cortex showed strong microglial activation similarly to that reported in amyloidogenic mice, the hippocampus of the same patients showed an attenuated microglial activation with a degenerative phenotype. Conclusions: These findings suggest the existence of different myeloid populations associated with Aβ plaques that correlates with disease severity. These results open the opportunity to design targeted therapies, not only to microglia, but also to the population of macrophages to modulate amyloid pathology and provide a better understanding of the immunological mechanisms underlying AD progression.
YR 2022
FD 2022
LK https://hdl.handle.net/10630/23883
UL https://hdl.handle.net/10630/23883
LA eng
NO Supported by ISCiii of Spain grants PI18/01557 (AG), PI18/01556 (JV) co-financed by FEDER funds from EU, by Junta de Andalucia grants UMA18-FEDERJA-211(AG), P18-RT-2233(AG) and US-1262734(JV) co-financed by Programa Operativo FEDER 2014-2020, and by B1-2019_07 Universidad de Malaga. Campus de Excelencia Internacional Andalucia Tech (ESM).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026