RT Conference Proceedings
T1 Depth profiling of tomato fruit cuticle during development using Confocal Raman Microscopy
A1 González Moreno, Ana
A1 Domínguez Carmona, Eva María
A1 Mayer, Konrad
A1 Xiao, Nannan
A1 Bock, Peter
A1 Heredia-Bayona, Antonio
A1 Gierlinger, Notburga
K1 Tomates - Investigación - Congresos
K1 Espectroscopía Raman - Congresos
AB The plant cuticle is defined as the first barrier between the aerial non-lignified organs of land plants and the environment. Some pivotal roles are ascribed to this outermost layer such as acting as support to the plant, protecting it against radiation, pathogens, water loss or mechanical injuries as well as controlling the gas exchange with the surroundings or the proper development of the plant. Although the composition of tomato fruit cuticle has been analysed, the spatial distribution of its components has received little attention.Confocal Raman Microscopy has been employed to locate the microspatial distribution and changes throughout development of different tomato fruit cuticle components. Results have shown the cutin matrix as a homogeneous layer. A combination of uni and multivariate approaches has allowed to selective locate the phenolic acids and flavonoids. Comparison of the cuticle Raman spectra with a reference library has additionally allowed to identify esterified p-coumaric acid and free p-hydroxybenzoic acid as the main phenolic acids present during growth. Both showed a similar distribution during fruit development, mainly located to the outermost layer of the cuticle and within pegs, which changed to a more homogenous distribution of esterified p-coumaric acid during ripening. Changes in flavonoid composition and location were also detected during ripening.Based on the results, we propose three models of spatial location of cuticle components during fruit development. During the earliest stages of development phenolics are homogenously distributed along the whole depth of the cuticle. Further in development, phenolics are restricted to the outermost side of the cuticle and the middle region of the pegs. Finally, during ripening, phenolic acids are located across the entire cuticle and flavonoids started to accumulate displaying a heterogeneous distribution along cuticle width, with a higher accumulation to the inner side.
YR 2022
FD 2022-09-12
LK https://hdl.handle.net/10630/25156
UL https://hdl.handle.net/10630/25156
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.Proyecto TI2018-094277-B/AEI/10.13039/501100011033 de la Agencia Estatal de Investigación, Ministerio de Ciencia e Innovación, Spain cofinnciado por la European Regional Development Fund (ERDF).Ana González es beneficiario de la beca con referencia FPU17/0177.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026