RT Journal Article
T1 Retrieval of germinal zone neural stem cells from the cerebrospinal fluid of premature infants with intraventricular hemorrhage
A1 Fernández-Muñoz, Beatriz
A1 Rosell-Valle, Cristina
A1 Ferrari, Daniela
A1 Alba-Amador, Julia
A1 Montiel, Miguel Ángel
A1 Campos-Cuerva, Rafael
A1 López-Navas, Luis
A1 Muñoz-Escalona, María
A1 Martín-López, María
A1 Profico, Daniela Celeste
A1 Blanco, Manuel Francisco
A1 Giorgetti, Alessandra
A1 González-Muñoz, María Elena
A1 Márquez-Rivas, Javier
A1 Sánchez Pernaute, Rosario
K1 Células madre neurales
K1 Niños prematuros
K1 Hemorragias
AB Intraventricular hemorrhage is a common cause of morbidity and mortality in prematureinfants. The rupture of the germinal zone into the ventricles entails loss of neural stem cellsand disturbs the normal cytoarchitecture of the region, compromising lateneurogliogenesis. Here we demonstrate that neural stem cells can be easily and robustlyisolated from the hemorrhagic cerebrospinal fluid obtained during therapeuticneuroendoscopic lavage in preterm infants with severe intraventricular hemorrhage. Ouranalyses demonstrate that these neural stem cells, although similar to human fetal celllines, display distinctive hallmarks related to their regional and developmental origin in thegerminal zone of the ventral forebrain, the ganglionic eminences that give rise to interneurons and oligodendrocytes. These cells can be expanded, cryopreserved, and differentiated in vitro and in vivo in the brain of nude mice and show no sign of tumoraltransformation 6 months after transplantation. This novel class of neural stem cells posesno ethical concerns, as the fluid is usually discarded, and could be useful for the development of an autologous therapy for preterm infants, aiming to restore late neurogliogenesisand attenuate neurocognitive deficits. Furthermore, these cells represent a valuable toolfor the study of the final stages of human brain development and germinal zone biology
PB Oxford University Press
YR 2020
FD 2020-05-30
LK https://hdl.handle.net/10630/38842
UL https://hdl.handle.net/10630/38842
LA eng
NO Beatriz Fernández-Muñoz, Cristina Rosell-Valle, Daniela Ferrari, Julia Alba-Amador, Miguel Ángel Montiel, Rafael Campos-Cuerva, Luis Lopez-Navas, María Muñoz-Escalona, María Martín-López, Daniela Celeste Profico, Manuel Francisco Blanco, Alessandra Giorgetti, Elena González-Muñoz, Javier Márquez-Rivas, Rosario Sanchez-Pernaute, Retrieval of germinal zone neural stem cells from the cerebrospinal fluid of premature infants with intraventricular hemorrhage, Stem Cells Translational Medicine, Volume 9, Issue 9, September 2020, Pages 1085–1101, https://doi.org/10.1002/sctm.19-0323
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026