RT Conference Proceedings
T1 Oligodendrocyte metabolism throughout its differentiation: immunocytochemistry study and its impact in remyelination
A1 Gismero Rodríguez, Javier
A1 López-Villodres, Juan Antonio
A1 Escamilla-Sánchez, Alejandro
A1 García Díaz, Beatriz
A1 Rodríguez-Pérez, Luis Manuel
A1 Mercado-Sáenz, Silvia
A1 Ortega-Jiménez, María Victoria
A1 Arranz-Salas, Isabel María
A1 Peláez-González, Alberto Carlos
A1 Bermúdez-Flores, Diego Teófilo
K1 Fosforilación - Congresos
K1 Neuroglia - Congresos
K1 Desmielinización - Congresos
K1 Sistema nervioso - Degeneración - Congresos
AB Introduction: Oligodendrocytes (OL) role in demyelinating pathologies such as multiple sclerosis and other neurodegenerative diseases is only recently being subject of extensive research. While the genetic and molecular aspects have been thoroughly studied, their metabolism was overshadowed. In order to develop new therapies to promote remyelination of already damaged axons, we need to accurately describe how OL metabolism affects axon myelination and trophic support (1). The objective of this study is to obtain cytological evidence of the extent of both glycolytic metabolism and oxidative phosphorylation by immunocytochemistry throughout the development of OL.Methods: Oligodendroglia cells from post-natal mice cortices were obtained and cultured. A wide assortment of differentiation-stage-specific cell surface antigens, a glycolytic and an oxidative phosphorylation marker were combined in several immunofluorescences to study both metabolic pathways in each step of differentiation. Results: After analysing them under confocal microscopy and imaging software, we observed a constant upregulation of glycolytic metabolism throughout differentiation, while oxidative phosphorylation seemed to increase with differentiation to then decrease when oligodendrocytes achieved their final maturation stage. Conclusions: Therefore, oxidative phosphorylation may be crucial in the differentiation of precursors and glycolysis would thus be the preferred metabolic pathway for fully matured OL.[1] Rosko L. et al. Neuroscientist. 2019;25(4):334–43.
YR 2022
FD 2022-09-07
LK https://hdl.handle.net/10630/25148
UL https://hdl.handle.net/10630/25148
LA eng
NO Supported by UMA and IBIMA and funding from two ongoing projects: - ‘Modulation of oligodendrocyte metabolism via blood vessel remodelling as target to promote remyelination’ (funding by NEURATRIS). - ‘Blood vessel remodelling modulates remyelination by oligodendrocyte metabolic reprogramming’ (funding by Arsep Foundation).Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 26 ene 2026