RT Conference Proceedings
T1 The potential of the emerging RNAi strategy, called SIGS, in the control of Botrytis cinerea in horticultural crops
A1 López-Laguna, Alba
A1 Vielba-Fernández, Alejandra
A1 Pérez-García, Alejandro
A1 Fernández-Ortuño, Dolores
K1 Plantas - Enfermedades y plagas - Congresos
K1 Plantas - Resistencia a enfermedades y plagas - Aspectos genéticos - Congresos
K1 Botrytis cinerea - Congresos
AB Botrytis cinerea, the causal agent of the gray mold disease, is one of the main limiting factors ofhorticultural crops production worldwide, consuming up to 40% of fungicides in its control.However, this fungus has been categorized by FRAC (Fungicide Resistance Action Committee)as a phytopathogen with a high risk for fungicide resistance development, a fact that has beendemonstrated in our country. In addition, and according to the "farm to fork" strategy of therecent European Green Deal, the diversity of fungicides available to growers will be reduced by50% in 2030. For this reason, alternative control tools and molecules with fungicide activity are,more than ever, needed to control this important disease. In this study, we intend to check if theefficacy of the emerging RNA interference (RNAi) strategy, called "spray-induced genesilencing" (SIGS), could be a valid sustainable solution and an alternative to the use ofconventional fungicides for the control of B. cinerea. For this purpose, several double-strandedRNA (dsRNA) has been designed against targets genes involved in the virulence/pathogenicityof the fungus. Preliminary results, obtained in in vivo assays, indicated that the application ofdsRNAs significantly reduces the development of the fungus, demonstrating the potential of theSIGS technique for the control of B. cinerea. On the other hand, and to improve the applicationof these oligonucleotides in the field, their encapsulation to create nanoparticles will be carriedout. If we succeed, new molecules with fungicidal action, could be included into the severalstrategies carried out to obtain a sustainable plant protection control programs in the field
YR 2022
FD 2022-09
LK https://hdl.handle.net/10630/25125
UL https://hdl.handle.net/10630/25125
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 24 ene 2026