RT Journal Article
T1 Single-cell in situ mapping of glioblastoma and astrocyte cell lines treated with a carbon dot–mediated riluzole nanotherapeutic agent: a live-cell µFTIR and soft X-ray tomography approach
A1 Dučić, Tanja
A1 Pereiro, Eva
A1 Ninkovic, Milena
A1 Sperling, Swetlana
A1 Rohde, Veit
A1 Fernández-González, Claudia
A1 Algarra, Manuel
A1 González-Muñoz, Elena
K1 Glioma
AB Nanoparticle-based drug carriers offer a promising alternative to conventional cancer therapies by enabling targeted delivery and reducing off-target toxicity. Here, we used synthesised and characterised carbon-based nanoparticles derived from 2-acrylamido-2-methylpropanesulfonic acid (AMPS-CDs), demonstrating biocompatibility with both human astrocytes and glioblastoma cells. We assessed their potential to enhance riluzole’s efficacy through synergistic interaction (AMPS-CDs@RZ) using live-cell synchrotron-based FTIR spectroscopy and cryo-soft X-ray tomography to monitor biochemical and structural changes at the single-cell level. While AMPS-CDs nanoparticles alone were non-toxic, the combination with riluzole significantly enhanced cell death in glioblastoma cells, with a significantly lower impact in non-cancerous astrocytes. Treatment with AMPS-CDs@RZ induced significant changes in bio-macromolecules, including DNA, protein conformation, and lipid metabolism. Notably, the treatment triggered nuclear envelope (NE) blebbing in glioblastoma cells, likely due to the interaction of the nanoparticle formulation with the nuclear membrane. This initiated stress signals that disrupted the cell’s inner intracellular membrane system, including the endoplasmic reticulum and mitochondria. To our knowledge, this is the first report linking NE blebbing to this mechanism involving membrane disassembly and nuclear envelope blebbing in riluzole-induced toxicity in glioblastoma is novel, providing a new therapeutic strategy and insights into cellular stress responses. These findings suggest that AMPS-CDs nanoparticles are a promising carrier for riluzole, potentially enhancing the specificity and efficacy of glioblastoma treatments while minimising damage to healthy tissues.
PB BMC Springer Nature
YR 2025
FD 2025
LK https://hdl.handle.net/10630/40325
UL https://hdl.handle.net/10630/40325
LA eng
NO Dučić, T., Pereiro, E., Ninkovic, M. et al. Single-cell in situ mapping of glioblastoma and astrocyte cell lines treated with a carbon dot–mediated riluzole nanotherapeutic agent: a live-cell µFTIR and soft X-ray tomography approach. J Nanobiotechnol 23, 629 (2025). https://doi.org/10.1186/s12951-025-03687-2
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026