RT Journal Article
T1 Structural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits.
A1 Orozco-Navarrete, Begoña
A1 Kaczmarska, Zuzanna
A1 Dupeux, Florine
A1 Garrido-Arandia, María
A1 Pott, Delphine
A1 Díaz-Perales, Araceli
A1 Casañal, Ana
A1 Márquez, José A.
A1 Valpuesta-Fernández, Victoriano
A1 Merchante-Berg, Catharina
K1 Fresas
K1 Alergenos
AB Although strawberries are highly appreciated fruits, their intake can induce allergic reactions in atopic patients. These reactions can be due to the patient’s previous sensitization to the major birch pollen allergen Bet v 1, by which IgE generated in response to Bet v 1 cross-reacts with the structurally related strawberry Fra a 1 protein family. Fra a 1.02 is the most expressed paralog in ripe strawberries and is highly allergenic. To better understand the molecular mechanisms regulating this allergic response, we have determined the three-dimensional structure of Fra a 1.02 and four site-directed mutants that were designed based on their positions in potential epitopes. Fra a 1.02 and mutants conform to the START fold. We show that the cross-reactivity of all the mutant variants to IgE from patients allergic to Bet v 1 was significantly reduced without altering the conserved structural fold, so that they could potentially be used as hypoallergenic Fra a 1 variants for the generation of vaccines against strawberry allergy in atopic patients.
PB American Chemical Society
YR 2019
FD 2019-11-27
LK https://hdl.handle.net/10630/29613
UL https://hdl.handle.net/10630/29613
LA eng
NO Orozco-Navarrete B, Kaczmarska Z, Dupeux F, Garrido-Arandia M, Pott D, Perales AD, Casañal A, Márquez JA, Valpuesta V, Merchante C. Structural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits. J Agric Food Chem. 2020 Sep 30;68(39):10951-10961. doi: 10.1021/acs.jafc.9b05714. Epub 2020 Jan 3. PMID: 31774998; PMCID: PMC7644122.
NO This work was supported by the following grants: a BIO2013-44199-R (MEC, Spain) to V.V., the Ramón y Cajal program RYC-2017-22323 (MINECO, Universidad de Málaga, Spain) to C.M., a BES-2014-068723 predoctoral fellowship to B.O.-N., a European Community’s Seventh Framework Programme H2020 under iNEXT (H2020 Grant #653706) to J.A.M., and a BIO2017-84548-R (MINECO, Spain) and Instituto de Salud Carlos III (ISCIII) cofounded by FEDER Thematic Networks and Cooperative Research Centers: ARADYAL (RD16/006/003) to A.D.P.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026