RT Journal Article
T1 Allelic Variation of MYB10 is the Major Force Controlling Natural Variation of Skin and Flesh Color in Strawberry (Fragaria spp.) fruit
A1 Castillejo, Cristina
A1 Waurich, Veronika
A1 Wagner, Henning
A1 Ramos, Rubén
A1 Oiza, Nicolás
A1 Muñoz, Pilar
A1 Triviño, Juan C
A1 Caruana, Julie
A1 Liu, Zhongchi
A1 Cobo, Nicolás
A1 Hardigan, Michael A
A1 Knapp, Steven J
A1 Vallarino Castro, José G.
A1 Osorio-Algar, Sonia
A1 Martín-Pizarro, Carmen
A1 Posé-Padilla, David
A1 Toivainen, Tuomas
A1 Hytönen, Timo
A1 Oh, Youngjae
A1 Barbey, Christopher R
A1 Whitaker, Vance M
A1 Lee, Seonghee
A1 Olbricht, Klaus
A1 Sánchez-Sevilla, José F
A1 Amaya Saavedra, Iraida
K1 Fresas - Variedades
AB Anthocyanins are the principal color-producing compounds synthesized in developing fruits of strawberry (Fragaria spp.).  Substantial natural variation in color have been observed in fruits of diploid and octoploid accessions, resulting from distinct accumulation and distribution of anthocyanins in fruits. Anthocyanin biosynthesis is controlled by a clade of R2R3 MYB transcription factors, among which MYB10 has been shown as the main activator in strawberry fruit. Here, we show that MYB10 mutations cause most of the anthocyanin variation observed in diploid woodland strawberry (F. vesca) and octoploid cultivated strawberry (F. ×ananassa). Using a mapping-by-sequencing approach, we identified a gypsytransposon insertion in MYB10 that truncates the protein and knocks out anthocyanin biosynthesis in a white-fruited F. vesca ecotype. Two additional lossof-function MYB10 mutations were identified among geographically diverse whitefruited F. vesca ecotypes. Genetic and transcriptomic analyses in octoploid Fragaria spp. revealed that FaMYB10-2, one of three MYB10 homoeologs identified, residing in the F. iinumae-derived subgenome, regulates the biosynthesis of anthocyanins in developing fruit. Furthermore, independent mutations in MYB10-2 are the underlying cause of natural variation in fruit skin and flesh color in octoploid strawberry. We identified a CACTA-like transposon (FaEnSpm-2) insertion in the MYB10-2 promoter of red-fleshed accessions that was associated with enhanced expression and anthocyanin accumulation. Our findings suggest that putative cis regulatory elements provided by FaEnSpm-2 arerequired for high and ectopic MYB10-2 expression and induction of anthocyanin biosynthesis in fruit flesh. We developed MYB10-2 (sub-genome) specific DNA markers for marker-assisted selection that accurately predicted anthocyanin phenotypes in octoploid segregating populations.
YR 2020
FD 2020-06-13
LK https://hdl.handle.net/10630/23562
UL https://hdl.handle.net/10630/23562
LA eng
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026