RT Journal Article
T1 Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica
A1 Pérez-Caballero, Raúl
A1 Siles-Lucas, Mar
A1 González-Miguel, Javier
A1 Martínez-Moreno, Francisco Javier
A1 Escamilla-Sánchez, Alejandro
A1 Pérez, José
A1 Martinez Moreno, Álvaro
A1 Buffoni, Leandro
K1 Inmunopatología
AB In this study, the immunogenicity and protective capacity of a new recombinant vaccine candidate, the rFh14-3-3z protein was analysed in sheep experimentally challenged with Fasciola hepatica, in terms of fluke burden, faecal egg counts, hepatic damage and humoral immune response. Three groups of 8 animals each were used for study, group 1 was immunised with the rFh14-3-3z in Montanide adjuvant, whereas group 2 and 3 remained as adjuvant control and infection control groups, respectively. The parasitological analysis showed that no significant reduction in fluke burden, fluke size and faecal egg counts was detected. The extent of hepatic damage was very similar between groups. Nonetheless, animals immunised with the rFh14-3-3z protein induced the development of specific IgG1 and IgG2, being the IgG1 the predominant antibody; which confirms the immunogenicity of this protein in sheep. This is the first report of the 14-3-3z proteins as vaccine against the infection with F. hepatica.
PB Elsevier B.V.
YR 2018
FD 2018-08
LK https://hdl.handle.net/10630/29231
UL https://hdl.handle.net/10630/29231
LA eng
NO Pérez-Caballero R, Siles-Lucas M, González-Miguel J, Martínez-Moreno FJ, Escamilla A, Pérez J, Martínez-Moreno A, Buffoni L. Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica. Vet Immunol Immunopathol. 2018 Aug;202:115-121. doi: 10.1016/j.vetimm.2018.07.006. Epub 2018 Jul 7. PMID: 30078585.
NO Finaciación de los siguientes Proyectos Nacionales: AGL2002-00644, AGL2015-67023-C2-1-R/2-R del Ministerio de Economía, Industria y Competitividad del Gobierno de España.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026