RT Conference Proceedings
T1 Peripheral membrane TTL proteins safeguard cellulose synthesis under stress.
A1 Amorim-Silva, Vitor
A1 García-Moreno, Álvaro
A1 Kesten, Christopher
A1 Menna, Alexandra
A1 Castillo-Garriga, Araceli
A1 Percio-Vargas, Francisco
A1 Pagano Márquez, Raquel
A1 Moya-Cuevas, José
A1 Armengot, Laia
A1 Ruiz-López, Noemí
A1 Jallais, Yvon
A1 Sánchez-Rodríguez, Clara
A1 Botella-Mesa, Miguel Ángel
K1 Celulosa - Síntesis
K1 Plantas - Efectos del estrés
AB Land plants provide around eighty percent of biomass on Earth and roughly one-third corresponds to cellulose (Bar-On et al 2018). Despite its biological and societal importance, many aspects of cellulose biosynthesis and regulation remain elusive. Controlled primary cell wall remodeling allows plant growth under stressful conditions, but how these changes are conveyed to adjust cellulose synthesis is not well understood (Colin et al 2023). In this work, we identify that Tetratricopeptide Thioredoxin-Like (TTL) proteins, which we previous describe as a scaffold of brassinosteroids signalling components, are also new members of the cellulose synthase complex (CSC) and we describe their unique and hitherto unknown dynamic association with the CSC under cellulose-deficient conditions (Amorim-Silva et al 2019 and Kesten, García-Moreno, Amorim-Silva et al 2022). We found out that TTLs are essential for maintaining cellulose synthesis under high salinity conditions, establishing a stress-resilient cortical microtubule array, and stabilizing CSCs at the plasma membrane. To fulfill these functions, TTLs interact with Cellulose Synthase1 (CESA1) and engage with cortical microtubules to promote their polymerization. We propose that TTLs function as bridges connecting stress perception with dynamic regulation of cellulose biosynthesis at the plasma membrane. In addition, we are currently working to identify and characterize new components involved in TTLs function and dynamics during cellulose biosynthesis under saline stress conditions.References:Amorim-Silva et al. 2019 The Plant CellBar-On et al. 2018 Proc. Natl. Acad. Sci.Colin et al. 2023 The Plant CellKesten, García-Moreno, Amorim-Silva et al. 2022 Sci. Adv.
YR 2023
FD 2023
LK https://hdl.handle.net/10630/27301
UL https://hdl.handle.net/10630/27301
LA eng
NO Meeting attendance was supported by Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. This work was funded by the Spanish Ministry for Science and Innovation (MCIN/AEI/ 10.13039/501100011033) (PGC2018-098789-B-I00)  and (PID2019-107657RB-C22) to MAB, NRL and AC respectively. The Andalusian Research Plan co-financed by the European Union (PAIDI 2020-PY20_00084) to MAB andde Andalucía UMA-FEDER project (grant UMA18-FEDERJA-154) to NRL, and the Swiss National foundation to CSR (SNF 31003A_163065/1 to AM). CK was supported by a Peter und Traudl Engelhorn-Stiftung fellowship, an ETH Career Seed Grant (SEED-05 19-2) of the ETH Foundation, an Emerging Investigator grant (NNF20OC0060564) of the Novo Nordisk Foundation, and an Experiment grant (R346-2020-1546) of the Lundbeck foundation. AGM and FP were supported by BES-2015-071256 and FPU19/02219 fellowships respectively.  VAS was supported by an Emerging Investigator research project (UMA20-FEDERJA-007) and co-financed by the “Programa Operativo FEDER 2014-2020” and by the “Consejería de Economía y Conocimiento de la Junta de Andalucía”..
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026