RT Conference Proceedings T1 Overestimation of Proteome Provides Insights into Transposable Element Landscape in Podosphaera xanthii, Causal Agent of Cucurbit Powdery Mildew Disease. A1 Jiménez Sánchez, Alejandro A1 Polonio Escalona, Alvaro Acisclo A1 Fernández-Ortuño, Dolores A1 Pérez-García, Alejandro K1 Hongos fitopatógenos K1 Oídio K1 Cucurbitáceas - Enfermedades y plagas AB Cucurbit powdery mildew, caused by Podosphaera xanthii, is one of the most destructive plant diseases of cucurbit crops worldwide. To date, there are discrepancies in the number of genes in the available genomic resources of the pathogen. We previously reported a predicted gene set of 16,030 in the P. xanthii isolate 2086, which was higher than that commonly described in other obligate biotrophic ascomycetes. The evaluation of the resulting proteome revealed approximately 7,000 consistent proteins and a surprising number of 8,940 unknown proteins. The study of this “dark” proteome showed that it actually originated from divergent transposable elements (TEs). For its part, the P. xanthii – Cucumis melo dual RNA-seq analysis showed a significant activity of some of these TEs during infection. Refining genome annotation taking into consideration repetitive elements could becritical for future metabolic and evolutionary studies aimed at understanding pathogen-host interactions. YR 2025 FD 2025-03 LK https://hdl.handle.net/10630/38203 UL https://hdl.handle.net/10630/38203 LA spa DS RIUMA. Repositorio Institucional de la Universidad de Málaga RD 12 abr 2026