RT Journal Article
T1 Vectors and P64k gene targeting for tandem affinity purification in Neisseria meningitidis
A1 Borroto Escuela, Dasiel Óscar
A1 Alea, Mileydis Perez
A1 Fernandez, Wilber Romero
A1 Gil, Daniel Bello
K1 Bacterias
K1 Meningitis
AB We present and describe the construction of tagging cassettes and plasmids for tandem affinity purification (TAP) of proteins in Neisseria meningitidis. The tagging cassette is designed for carboxyl-terminal tagging of proteins and it contains only two repeats of IgG-binding units. P64k protein from N. meningitidis was chosen to fuse at these new affinity tags. This protein is well recognized in immunoassays by serum from human convalescent meningococcal disease and it is highly immunogenic in animals. To continue the characterization of this meningococcal antigen, we designed and constructed two vectors for use in TAP purification method. We also carried-out preliminary test to check the correct expression of the protein fused in these vectors.
PB Elsevier
SN 01677012
YR 2006
FD 2006-04-01
LK https://hdl.handle.net/10630/45003
UL https://hdl.handle.net/10630/45003
LA eng
NO Dasiel Oscar Borroto Escuela, Mileydis Perez Alea, Wilber Romero Fernandez, Daniel Bello Gil, Vectors and P64k gene targeting for tandem affinity purification in Neisseria meningitidis, Journal of Microbiological Methods, Volume 65, Issue 1, 2006, Pages 187-193, ISSN 0167-7012, https://doi.org/10.1016/j.mimet.2005.07.013. (https://www.sciencedirect.com/science/article/pii/S0167701205001958)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026