RT Journal Article
T1 A lysin motif effector subverts chitin-triggered immunity to facilitate arbuscular mycorrhizal symbiosis
A1 Zeng, Tian
A1 Rodríguez-Moreno, Luis Gabriel
A1 Mansurkhodzaev, Artem
A1 Wang, Peng
A1 van den Berg, Willy
A1 Gasciolli, Virginie
A1 Cottaz, Sylvain
A1 Fort, Sébastien
A1 Thomma, Bart
A1 Bono, Jean-Jaques
A1 Bisseling, Ton
A1 Limpens, Erik
K1 Micorrizas
K1 Simbiosis
K1 Inmunología vegetal
AB Arbuscular mycorrhizal (AM) fungi greatly improve mineral uptake by host plants in nutrient-depleted soil and can intracellularly colonize root cortex cells in the vast majority of higher plants. However, AM fungi possess common fungal cell wall components such as chitin that can be recognized by plant chitin receptors to trigger immune responses, raising the question as to how AM fungi effectively evade chitin-triggered immune responses during symbiosis. In this study, we characterize a secreted lysin motif (LysM) effector identified from the model AM fungal species Rhizophagus irregularis, called RiSLM. RiSLM is one of the highest expressed effector proteins in intraradical mycelium during the symbiosis. In vitro binding assays show that RiSLM binds chitin-oligosaccharides and can protect fungal cell walls from chitinases. Moreover, RiSLM efficiently interferes with chitin-triggered immune responses, such as defence gene induction and reactive oxygen species production in Medicago truncatula. Although RiSLM also binds to symbiotic (lipo)chitooligosaccharides it does not interfere significantly with symbiotic signalling in Medicago. Host-induced gene silencing of RiSLM greatly reduces fungal colonization levels. Taken together, our results reveal a key role for AM fungal LysM effectors to subvert chitin-triggered immunity in symbiosis, pointing to a common role for LysM effectors in both symbiotic and pathogenic fungi.
PB Wiley
YR 2020
FD 2020-01
LK https://hdl.handle.net/10630/29863
UL https://hdl.handle.net/10630/29863
LA eng
NO Zeng, T., Rodriguez-Moreno, L., Mansurkhodzaev, A., Wang, P., van den Berg, W., Gasciolli, V., Cottaz, S., Fort, S., Thomma, B.P.H.J., Bono, J.-J., Bisseling, T. and Limpens, E. (2020), A lysin motif effector subverts chitin-triggered immunity to facilitate arbuscular mycorrhizal symbiosis. New Phytol, 225: 448-460. https://doi.org/10.1111/nph.16245
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026