RT Journal Article
T1 Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein
A1 Nieto-Torres, Jose Luis
A1 López-De-Diego, Marta
A1 Álvarez-Coruña, Enrique
A1 Jimenez-Guardeño, Jose Manuel
A1 Regla-Nava, Jose Angel
A1 Llorente-Gómez, Mercedes
A1 Kremer, Leonor
A1 Shuo, Shen
A1 Enjuanes, Luis
K1 Coronavirus
K1 COVID-19
AB Severe acute respiratory syndrome (SARS) coronavirus (CoV) envelope (E) protein is a transmembrane protein. Several subcellular locations and topological conformations of E protein have been proposed. To identify the correct ones, polyclonal and monoclonal antibodies specific for the amino or the carboxy terminus of E protein, respectively, were generated. E protein was mainly found in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of cells transfected with a plasmid encoding E protein or infected with SARS-CoV. No evidence of E protein presence in the plasma membrane was found by using immunofluorescence, immunoelectron microscopy and cell surface protein labeling. In addition, measurement of plasma membrane voltage gated ion channel activity by whole-cell patch clamp suggested that E protein was not present in the plasma membrane. A topological conformation in which SARS-CoV E protein amino terminus is oriented towards the lumen of intracellular membranes and carboxy terminus faces cell cytoplasm is proposed.
PB Elsevier
YR 2011
FD 2011
LK https://hdl.handle.net/10630/36919
UL https://hdl.handle.net/10630/36919
LA spa
NO Jose L. Nieto-Torres, Marta L. DeDiego, Enrique Álvarez, Jose M. Jiménez-Guardeño, Jose A. Regla-Nava, Mercedes Llorente, Leonor Kremer, Shen Shuo, Luis Enjuanes, Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein, Virology, Volume 415, Issue 2, 2011, Pages 69-82, ISSN 0042-6822, https://doi.org/10.1016/j.virol.2011.03.029
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 28 feb 2026