RT Journal Article
T1 Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence.
A1 Gémez Mata, Juan
A1 Souto, Sandra
A1 Bandin, Isabel
A1 Alonso-Sánchez, María del Carmen
A1 Borrego-García, Juan José
A1 Labella Vera, Alejandro Manuel
A1 García-Rosado, Esther
K1 Virus con ARN
K1 Lenguados
AB Nervous necrosis virus (NNV), genus Betanodavirus, the etiological agent of the viral encephalopathyand retinopathy (VER), presents a genome with two positive-sense single-strandedRNA segments. Striped jack nervous necrosis virus (SJNNV) and red-spotted grouper nervousnecrosis virus (RGNNV), together with reassortants RGNNV/SJNNV, are the betanodaviruses predominantlyisolated in Southern Europe. An RGNNV/SJNNV reassortant isolated from Senegalesesole (wt160) causes high mortalities in this fish species. This virus presents differences in the sequenceof the 3’ non-coding region (NCR) of both segments compared to RGNNV and SJNNV referencestrains. Previously, it has been reported that the reversion of two of these differences (nucleotides 1408and 1412) in the RNA2 3’NCR to the SJNNV-type (recombinant r1408-1412) resulted in a decrease insole mortality. In the present study, we have applied an OpenArray® to analyse the involvement ofsole immune response in the virulence of several recombinants: the r1408-1412 and two recombinants,developed in the present study, harbouring mutations at positions 3073 and 3093 of RNA1 3’NCR torevert them to RGNNV-type. According to the correlation values and to the number of expressedgenes, the infection with the RNA2-mutant provoked the most different immune response comparedto the immune response triggered after the infection with the rest of the viruses, and the exclusive andhigh upregulation of genes related to the complement system. The infection with the RNA1-mutantsalso provoked a decrease in mortality and their replication was delayed at least 24 h compared to thewt160 replication, which could provoke the lag observed in the immune response. Furthermore, theinfection with the RNA1-mutants provoked the exclusive expression of pkr and the downregulationof il17rc.
PB MDPI
YR 2021
FD 2021-10-27
LK https://hdl.handle.net/10630/34427
UL https://hdl.handle.net/10630/34427
LA eng
NO Gémez-Mata J, Souto S, Bandín I, Alonso MDC, Borrego JJ, Labella AM, García-Rosado E. Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence. Pathogens. 2021 Oct 27;10(11):1388. doi: 10.3390/pathogens10111388. PMID: 34832544; PMCID: PMC8621919.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026