RT Journal Article
T1 Biological Effects of Polysaccharides from Bovistella utriformis  as Cytotoxic, Antioxidant, and Antihyperglycemic Agents:  In Vitro and In Vivo Studies.
T2 MDPI
A1 Maaloul, Aya
A1 Pérez Manríquez, Claudia
A1 Decara, Juan
A1 Mari-Beffa, Manuel
A1 Álvarez-Torres, Daniel
A1 Latorre-Redoli, Sofía
A1 Martínez-Albardonedo, Borja
A1 Araya-Rojas, Marisel
A1 Fajardo, Víctor
A1 Abdala-Díaz, Roberto Teófilo
K1 Hongos medicinales
K1 Polisacáridos
K1 Antioxidantes
K1 Peces cebra - Toxicidad
K1 Citotoxicidad por mediación celular
K1 Hipoglucemiantes
K1 Biomasa - Composición
AB This study explores the bioactive potential of Bovistella utriformis biomass and its polysaccharides (PsBu) through comprehensive biochemical and bioactivity analyses, focusing on their antioxidant, cytotoxic, and antihyperglycemic properties. Methods: Elemental analysis determined the biomass’s chemical composition. Antioxidant activity was assessed using ABTS and DPPH assays. Monosaccharide composition was analyzed via gas chromatography-mass spectrometry (GC-MS). In vitro cytotoxicity assays were conducted on cancer and normal cell lines to determine IC50 values and selectivity indices (SI). Zebrafish embryo toxicity was evaluated for teratogenic effects, and an in vivo antihyperglycemic study was performed in diabetic rat models. Results: The biomass exhibited high carbon content (around 41%) and nitrogen levels, with a balanced C/N ratio nearing 5. Protein content exceeded 50%, alongside significant carbohydrate, fiber, and ash levels. Antioxidant assays revealed inhibition rates of approximately 89% (ABTS) and 64% (DPPH). GC-MS analysis identified glucose as the predominant sugar (>80%), followed by galactose and mannose. Additionally, HPLC detected a photoprotective compound, potentially a mycosporin-like amino acid. Cytotoxicity assays demonstrated PsBu’s selective activity against colon, lung, and melanoma cancer cell lines (IC50: 100–500 µg·mL−1), while effects on normal cell lines were lower (IC50 > 1300 µg·mL−1 for HaCaT, >2500 µg·mL−1 for HGF-1), with SI values approaching 27, supporting PsBu’s potential as a targeted anticancer agent. Zebrafish embryo assays yielded LC50 values ranging from 1.4 to 1.8 mg·mL−1.
PB MDPI
YR 2025
FD 2025-03-05
LK https://hdl.handle.net/10630/40112
UL https://hdl.handle.net/10630/40112
LA eng
NO Maaloul, A., Pérez Manríquez, C., Decara, J., Marí-Beffa, M., Álvarez-Torres, D., Latorre Redoli, S., Martínez-Albardonedo, B., Araya-Rojas, M., Fajardo, V., & Abdala Díaz, R. T. (2025). Biological Effects of Polysaccharides from Bovistella utriformis as Cytotoxic, Antioxidant, and Antihyperglycemic Agents: In Vitro and In Vivo Studies. Pharmaceutics, 17(3), 335. https://doi.org/10.3390/pharmaceutics17030335
NO Junta de Andalucía
NO NextGenerationEU
NO Sistema Nacional de Salud
NO ISCIII
NO European Social Fund
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026