RT Conference Proceedings
T1 CRISPR/Cas9-mediated editing of the TM6 MADS-box gene in the octoploid strawberry (Fragaria x ananassa)
A1 Martín-Pizarro, Carmen
A1 Posé-Padilla, David
K1 Ingeniería genética vegetal
AB The B-class of MADS-box transcription factors has been studied in many plant species, butremain functionally uncharacterized in the Rosaceae family. APETALA3 (AP3), a member of thisclass, controls the identity of petals and stamens in Arabidopsis thaliana. In this work, we identifiedtwo members of the AP3 lineage in the cultivated strawberry (Fragaria × ananassa): FaAP3and FaTM6. Interestingly, FaTM6, and not FaAP3, shows an expression pattern equivalent to thatof AP3 in Arabidopsis. Genome editing using Cluster Regularly Interspaced Short PalindromicRepeats (CRISPR)/Cas9 system is becoming a robust tool for targeted and stable mutagenesisof DNA. However, whether it can be efficiently used in an octoploid species such as F. × ananassais not known. In our study, we report the application of the CRISPR/Cas9 in F. × ananassa tocharacterize the function of FaTM6 in flower development. An exhaustive analysis by high-throughputsequencing of the FaTM6 locus spanning the target sites showed a high efficiency genomeediting already in the T0 generation. The phenotypic characterization of the mutant linesindicates that FaTM6 plays a key role in petal and especially in anther development in strawberry.Our results validate the CRISPR/Cas9 strategy for gene functional analysis in an octoploidspecies such as F. × ananassa, and offer new opportunities for engineering strawberry to improvetraits of interest in breeding programs.
YR 2019
FD 2019-05-10
LK https://hdl.handle.net/10630/17634
UL https://hdl.handle.net/10630/17634
LA eng
NO El contenido del poster presentado está desarrollado  en: doi:10.1093/jxb/ery400 yPublicado por Oxford University press
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 22 ene 2026