RT Conference Proceedings
T1 Human pluripotent stem cells as a research tool for elucidating the role of glial cells in Alzheimer´s disease
A1 García-León, Juan Antonio
A1 Cáceres Palomo, Laura
A1 Dávila-Cansino, José Carlos
A1 Vitorica Ferrández, Javier
A1 Gutiérrez-Pérez, Antonia
K1 Alzheimer, Enfermedad de
AB Background:Alzheimer's disease (AD) is characterized by presenting a complex pathology,not fully resolved yet. This fact, together with the lack of reliable models, hasimpeded the development of effective therapies. Recently, several studies haveshown that functional glial cell defects have a key role in the pathology of AD.However, this glial dysfunction, currently, cannot be correctly modeled usingthe available animal models, so we hypothesized that cells derived fromAlzheimer's patients can serve as a better platform for studying the disease. Inthis sense, human pluripotent stem cells (hPSC) allow the generation ofdifferent types of neural cells, which can be used for disease modeling,identification of new targets and drugs development.Methods:We have a collection of hiPSCs derived from patients with sporadic forms ofAD. We have differentiated these cells towards neural lineage to obtain neuronsand astrocytes. For the generation of oligodendrocytes (OLs), we havedeveloped a fast and robust protocol to generate mature OLs in just 22 days.Results:We have generated neural precursors from all the lines tested. In the case ofOLs, the cells generated resemble primary OLs and can myelinate neurons invivo and in vitro using a screening compatible platform. This platform is beingtransferred for the generation of the other glial cells.Conclusions:This methodology can be used to elucidate the pathogenic pathways associatedwith neurodegeneration and to identify new therapeutic targets susceptible tomodulation, contributing to the development of new effective drugs against AD.
YR 2020
FD 2020-12-18
LK https://hdl.handle.net/10630/20636
UL https://hdl.handle.net/10630/20636
LA eng
NO Acknowledgments:J.A.G.L has been supported by a contract of doctor reincorporation plan fromthe I Plan Propio of the University of Malaga (Spain) and by CIBERNED. Thisstudy was supported by Instituto de Salud Carlos III (ISCiii) of Spain, cofinancedby FEDER funds from European Union, through grants PI18/01557 (toAG), PI18/01556 (to JV), and CIBERNED (CB06/05/1116 to AG andCB06/05/0094 to JV); by Junta de Andalucia through Consejería de Economía yConocimiento grants UMA18-FEDERJA-211 (to AG), PY18-RT-2233 (to AG)and US-1262734 (to JV) co-financed by Programa Operativo FEDER 2014-2020 and Consejeria de Salud grant PI-0276-2018 (to JAGL).Acknowledgments:J.A.G.L has been supported by a contract of doctor reincorporation plan fromthe I Plan Propio of the University of Malaga (Spain) and by CIBERNED. Thisstudy was supported by Instituto de Salud Carlos III (ISCiii) of Spain, cofinancedby FEDER funds from European Union, through grants PI18/01557 (toAG), PI18/01556 (to JV), and CIBERNED (CB06/05/1116 to AG andCB06/05/0094 to JV); by Junta de Andalucia through Consejería de Economía yConocimiento grants UMA18-FEDERJA-211 (to AG), PY18-RT-2233 (to AG)and US-1262734 (to JV) co-financed by Programa Operativo FEDER 2014-2020 and Consejeria de Salud grant PI-0276-2018 (to JAGL).Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026