RT Conference Proceedings
T1 The arogenate dehydratase ADT2 is essential for seed development in Arabidopsis
A1 El-Azaz-Ciudad, Jorge
A1 Cánovas-Ramos, Francisco Miguel
A1 Ávila-Sáez, Concepción
A1 De-la-Torre-Fazio, Fernando Nicolás
K1 Fenilalanina - Síntesis
AB Phenylalanine (Phe) biosynthesis in plants is a key process, as Phe serves asprecursor of proteins and phenylpropanoids. The prephenate pathway connectschorismate, final product of the shikimate pathway, with the biosynthesis of Pheand Tyr. Two alternative routes of Phe biosynthesis have been reported: onedepending of arogenate, and the other of phenylpyruvate. Whereas the arogenatepathway is considered the main route, the role of the phenylpyruvate pathwayremains unclear. Here, we report that the deficiency in ADT2, a bifunctionalarogenate dehydratase (ADT)/ prephenate dehydratase (PDT) enzyme, causesembryo arrest and seed abortion. This result makes a clear distinction between theessential role of ADT2 and the five remaining ADTs from Arabidopsis, whichdisplay mostly overlapping functions. We have found that PHA2, a monofunctionalPDT from yeast, restores the adt2 phenotype when is targeted within the plastids,but not when is expressed in the cytosol. Similar results can be obtained byexpressing ADT3, a monofunctional ADT. These results suggest that Phe can besynthesized from phenylpyruvate or arogenate when the bifunctional ADT2 isreplaced by other ADT or PDT enzymes during seed formation, highlighting theimportance of Phe for embryo development, and providing further insights into theplasticity of Phe biosynthesis.
YR 2018
FD 2018-07-25
LK https://hdl.handle.net/10630/16352
UL https://hdl.handle.net/10630/16352
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026