RT Journal Article T1 Variations in storage protein and carbohydrate levels during development of avocado zygotic embryos T2 Variaciones en los niveles de proteínas y carbohidratos de almacenamiento durante el desarrollo de embriones cigóticos de aguacate A1 Sánchez-Romero, Carolina A1 Perán-Quesada, Rosa Amelia A1 Barcelo-Muñoz, Araceli A1 Pliego-Alfaro, Fernando K1 Aguacates AB Variations in carbohydrates and proteins were monitored during avocado (Persea americana Mill.) zygotic embryo development and correlated with growth parameters in order to define specific markers characterizing distinct embryogenic phases. Hexose (glucose and fructose) levels were initially high and declined as embryo development advanced reaching the lowest levels in completely mature embryos. Sucrose and starch evolution showed an opposite trend with a progressive increase during embryo growth. The beginning of the maturation phase could be identified by a switch in the carbohydrate status from high hexose/sucrose ratio to low hexose/sucrose ratio. Storage protein accumulation began at early cotyledonary stages (7–8 mm), increasing significantly in the maturation phase where they represented 83% of total proteins. Mature embryos (38–40 mm) contained albumins, globulins and glutelins, albumins being the predominant and most heterogeneous fraction. Storage protein accumulation occurred in a sequential and specific way suggesting a possible role as indicators of embryo development. The complete maturation stage could be characterized by the synthesis and accumulation of a 49 kDa albumin. PB Elsevier YR 2002 FD 2002 LK https://hdl.handle.net/10630/37336 UL https://hdl.handle.net/10630/37336 LA eng NO Carolina Sánchez-Romero, Rosa Perán-Quesada, Araceli Barceló-Muñoz, Fernando Pliego-Alfaro, 2002. Variations in storage protein and carbohydrate levels during development of avocado zygotic embryos, Plant Physiology and Biochemistry, Vol 40, Issue 12, Pp 1043-1049, ISSN 0981-9428. https://doi.org/10.1016/S0981-9428(02)01469-9. NO Según recoge la web de ELSEVIER: https://www.elsevier.com/about/policies-and-standards/sharing. Preprint: Authors can share their preprint anywhere at any time and If accepted for publication, we encourage authors to link from the preprint to their formal publication via its Digital Object Identifier (DOI). NO This work was supported by the Comision Interministerial de Ciencia y Tecnología, Spain (grant no. AGL 2000- 2003, C03-03). DS RIUMA. Repositorio Institucional de la Universidad de Málaga RD 21 ene 2026