RT Journal Article
T1 Postharvest changes in LIN5-down-regulated plants suggest a role for sugar deficiency in cuticle metabolism during ripening.
A1 Vallarino Castro, José G.
A1 Yeats, Trevor H.
A1 Maximova, Eugenia
A1 Rose, Jocelyn K.
A1 Fernie, Alisdair R.
A1 Osorio-Algar, Sonia
K1 Tomates - Metabolismo
K1 Plantas - Metabolismo
AB The cell wall invertase gene (LIN5) was reported to be a key enzyme influencing sugar uptake of tomato(Solanum lycopersicum) fruit. It was additionally revealed to be a key regulator of total soluble solidscontent in fruit as well as for reproductive development, being mainly involved in flower development,early fruit and seed development but also in ripening. Here, we demonstrate that silencing of the LIN5gene promotes changes affecting fruit cuticle development which has a direct effect on postharvestproperties. Transformants were characterized by reduced transpirational water loss in mature fruitsaccompanied by several other changes in the cuticle. Quantitative chemical composition, coupled withmicroscopy of isolated cuticle fruits revealed that the cuticle of the transformants were characterized byan increase of the thickness as well as significant increase in the content of cuticle components (cutin,phenolic compounds, and waxes). Furthermore, detailed analysis of the waxes revealed that the trans-formants displayed changes in waxes composition, showing higher levels of n-alkanes and triterpenoidswhich can shift the proportion of crystalline and amorphous waxes and change the water flux throughthe cuticle. Expression of the genes involved in cuticle biosynthesis indicated that LIN5 influences thebiosynthesis of components of the cuticle, indicating that this process is coupled to sugar uploading via amechanism which links carbon supply with the capacity for fruit expansion.
PB Elsevier
YR 2017
FD 2017
LK https://hdl.handle.net/10630/34526
UL https://hdl.handle.net/10630/34526
LA eng
NO https://v2.sherpa.ac.uk/id/publication/4667
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 22 ene 2026