RT Journal Article
T1 Extra spindle poles (Separase) controls anisotropic cell expansion in Norway spruce (Picea abies) embryos independently of its role in anaphase progression.
A1 Moschou, Panagiotis N.
A1 Savenkov, Eugene I
A1 Minina, Elena A.
A1 Fukada, Kazutake
A1 Reza, Salim Hossain
A1 Gutiérrez-Beltrán, Emilio
A1 Sánchez-Vera, Victoria
A1 Suárez-Marín, María Fernanda
A1 Hussey, Patrick J.
A1 Smertenko, Andrei
A1 Bozhkov, Peter V.
K1 Embriología vegetal
K1 Abetos rojos - Citología
AB The caspase-related protease separase (EXTRA SPINDLE POLES, ESP) plays a major role in chromatid disjunction and cell expansion in Arabidopsis thaliana. Whether the expansion phenotypes are linked to defects in cell division in Arabidopsis ESP mutants remains elusive.  Here we present the identification, cloning and characterization of the gymnosperm Nor-way spruce (Picea abies, Pa) ESP. We used the P. abies somatic embryo system and a combi-nation of reverse genetics and microscopy to explore the roles of Pa ESP during embryogenesis.  Pa ESP was expressed in the proliferating embryonal mass, while it was absent in the sus-pensor cells. Pa ESP associated with kinetochore microtubules in metaphase and then with anaphase spindle midzone. During cytokinesis, it localized on the phragmoplast microtubules and on the cell plate. Pa ESP deficiency perturbed anisotropic expansion and reduced mitotic divisions in cotyledonary embryos. Furthermore, whilst Pa ESP can rescue the chromatid nondisjunction phenotype of Arabidopsis ESP mutants, it cannot rescue anisotropic cell expan-sion.  Our data demonstrate that the roles of ESP in daughter chromatid separation and cell expansion are conserved between gymnosperms and angiosperms. However, the mechanisms of ESP-mediated regulation of cell expansion seem to be lineage-specific
PB Wiley-Blackwell
YR 2016
FD 2016-05-27
LK https://hdl.handle.net/10630/34985
UL https://hdl.handle.net/10630/34985
LA eng
NO https://openpolicyfinder.jisc.ac.uk/id/publication/15984
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026