RT Journal Article
T1 Identification of an Antigenic Determinant of Clavulanic Acid Responsible for IgE‐mediated Reactions
A1 Barbero, Nekane
A1 Fernández-Santamaría, Rubén
A1 Mayorga Mayorga, Cristobalina
A1 Martín-Serrano Ortiz, Ángela
A1 Salas, Maria
A1 Bogas, Gador
A1 Nájera-Albendín, Francisco
A1 Pérez-Sala, Dolores
A1 Perez-Inestrosa, Ezequiel
A1 Fernandez, Tahia D
A1 Montañez-Vega, María Isabel
A1 Torres, Maria J
K1 Alergia a los medicamentos
AB Background: Selective reactions to clavulanic acid (CLV) account for around 30% ofimmediate reactions after administration of amoxicillin‐CLV. Currently, no immunoas‐say is available for detecting specific IgE to CLV, and its specific recognition in pa‐tients with immediate reactions has only been demonstrated by basophil activationtesting, however with suboptimal sensitivity. The lack of knowledge regarding thestructure of the drug that remains bound to proteins (antigenic determinant) is ham‐pering the development of in vitro diagnostics. We aimed to identify the antigenicdeterminants of CLV as well as to evaluate their specific IgE recognition and potentialrole for diagnosis.Methods: Based on complex CLV degradation mechanisms, we hypothesized the for‐mation of two antigenic determinants for CLV, AD‐I (N‐protein, 3‐oxopropanamide)and AD‐II (N‐protein, 3‐aminopropanamide), and designed different synthetic analogsto each one. IgE recognition of these structures was evaluated in basophils from pa‐tients with selective reactions to CLV and tolerant subjects. In parallel, the CLV frag‐ments bound to proteins were identified by proteomic approaches.Results: Two synthetic analogs of AD‐I were found to activate basophils from allergicpatients. This determinant was also detected bound to lysines 195 and 475 of CLV‐treated human serum albumin. One of these analogs was able to activate basophils in59% of patients whereas CLV only in 41%. Combining both results led to an increasein basophil activation in 69% of patients, and only in 12% of controls.Conclusion: We have identified AD‐I as one CLV antigenic determinant, which is thedrug fragment that remains protein‐bound.
PB John Wiley and Sons Ltd.
YR 2019
FD 2019
LK https://hdl.handle.net/10630/36108
UL https://hdl.handle.net/10630/36108
LA eng
NO Identification of an Antigenic Determinant of Clavulanic Acid Responsible for IgE‐mediated Reactions Nekane Barbero, Rubén Fernández-Santamaría, Cristobalina Mayorga, Ángela Martin-Serrano, María Salas, Gador Bogas, Francisco Nájera, Dolores Pérez-Sala, Ezequiel Pérez-Inestrosa, Tahia D. Fernandez, María I. Montañez, María J. Torres Allergy, 2019, 74 (8), 1490-1501. DOI: 10.1111/all.13761
NO Institute of Health “Carlos III”Ministry of Economy and CompetitivenessEuropean Regional Development Fund (ERDF)Andalusian Regional Ministry of Economy and Knowledge
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 mar 2026