RT Journal Article
T1 Maladaptive stress-coping behavior in CX3CR1-deficient mice: Impact of adolescent stress and alcohol exposure on neuroimmune responses and inflammation
A1 Medina-Vera, Dina
A1 Martín-Chaves, Laura
A1 Sánchez-Marín, Laura
A1 Díaz-Ottaviano, María
A1 Gavito, Ana L.
A1 Popova, Olga
A1 Sánchez-Quintero, María José
A1 Rodríguez Capitán, Jorge
A1 Rodriguez-de-Fonseca, Fernando
A1 Jiménez-Navarro, Manuel Francisco
A1 Serrano, Antonia
A1 Pavón-Morón, Francisco Javier
K1 Ratones de laboratorio - Efectos del estrés
K1 Alcohol - Efectos fisiológicos
K1 Citoquinas
K1 Neurofarmacología
AB The CX3CL1/CX3CR1 chemokine axis regulates synaptic pruning, plasticity, and stress-related behaviors, influencing resilience or vulnerability to psychiatric disorders. Adolescence, a critical period for neuroimmune development, increases susceptibility to stressors. This study investigated how adolescent restraint stress and alcohol exposure affect stress-coping behavior, neuroimmune signaling, and systemic inflammation in adult wild-type (WT) and CX3CR1 knock-out (KO) mice.Eighty-one male and female WT and KO mice were assigned to control (non-stressed, saline-treated), stress (stressed, saline-treated), alcohol (non-stressed, alcohol-treated), and stress + alcohol (stressed, alcohol-treated) groups. Behavioral responses were evaluated using the tail suspension test. Hypothalamic gene expression of CX3CL1/CX3CR1, corticotropin-releasing hormone (CRH), and neuropeptide Y (NPY) systems was analyzed alongside plasma corticosterone, adrenocorticotropic hormone (ACTH), CX3CL1, and inflammatory mediators.Adolescent stress—but not alcohol—increased plasma CX3CL1 levels, which inversely correlated with immobility time in WT mice. KO mice displayed higher baseline immobility than WT mice, whereas stress and/or alcohol paradoxically reduced immobility. These behavioral effects were reproduced by pharmacological inhibition of CX3CR1. Additionally, KO mice showed disrupted hypothalamic expression of multiple genes in the CRH pathway and Npy1r, attenuated corticosterone responses to stress, and abolished ACTH–corticosterone correlation, suggesting HPA axis dysregulation. KO mice also exhibited exacerbated inflammatory responses to stress and alcohol, including elevated IL-17A/F, IL-11, and IFN-β1 levels.
PB Elsevier
YR 2025
FD 2025-05-12
LK https://hdl.handle.net/10630/38667
UL https://hdl.handle.net/10630/38667
LA eng
NO Medina-Vera, D., Martín-Chaves, L., Sánchez-Marín, L., Díaz-Ottaviano, M., Gavito, A. L., Popova, O., Sánchez-Quintero, M. J., Rodríguez-Capitán, J., Rodríguez de Fonseca, F., Jiménez-Navarro, M. F., Serrano, A., & Pavón-Morón, F. J. (2025). “Maladaptive stress-coping behavior in CX3CR1-deficient mice: Impact of adolescent stress and alcohol exposure on neuroimmune responses and inflammation.” Neuropharmacology, 275, 110503.
NO Funding for open access charge: Universidad de Málaga / CBUA
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026