RT Journal Article
T1 Regulation of L-ascorbic acid content in Strawberry fruits
A1 Cruz-Rus, Eduardo
A1 Amaya, Iraida
A1 Sánchez Sevilla, José
A1 Botella, Miguel Angel
A1 Valpuesta-Fernández, Victoriano
K1 Fresas - Aspectos moleculares
AB Plants have several L-ascorbic acid (AsA) biosynthetic pathways, but the contribution of each one to the synthesis of AsA varyies between different species, organs, and developmental stages. Strawberry (Fragaria×ananassa) fruits are rich in AsA. The pathway that uses D-galacturonate as the initial substrate is functional in ripe fruits, but the contribution of other pathways to AsA biosynthesis has not been studied. The transcription of genes encoding biosynthetic enzymes such as D-galacturonate reductase (FaGalUR) and myo-inositol oxygenase (FaMIOX), and the AsA recycling enzyme monodehydroascorbate reductase (FaMDHAR) were positively correlated with the increase in AsA during fruit ripening. Fruit storage for 72 h in a cold room reduced the AsA content by 30%. Under an ozone atmosphere, this reduction was 15%. Ozone treatment increased the expression of the FaGalUR, FaMIOX, and L-galactose-1-phosphate phosphatase (FaGIPP) genes, and transcription of the L-galactono-1,4-lactone dehydrogenase (FaGLDH) and FAMDHAR genes was higher in the ozone-stored than in the air-stored fruits. Analysis of AsA content in a segregating population from two strawberry cultivars showed high variability, which did not correlate with the transcription of any of the genes studied. Study of GalUR protein in diverse cultivars of strawberry and different Fragaria species showed that a correlation between GalUR and AsA content was apparent in most cases, but it was not general. Three alleles were identified in strawberry, but any sequence effect on the AsA variability was eliminated by analysis of the allele-specific expression. Taken together, these results indicate that FaGalUR shares the control of AsA levels with other enzymes and regulatory elements in strawberry fruit.
PB Oxford Academy
YR 2011
FD 2011-05-11
LK https://hdl.handle.net/10630/37427
UL https://hdl.handle.net/10630/37427
LA eng
NO Eduardo Cruz-Rus, Iraida Amaya, José F. Sánchez-Sevilla, Miguel A. Botella, Victoriano Valpuesta, Regulation of L-ascorbic acid content in strawberry fruits, Journal of Experimental Botany, Volume 62, Issue 12, August 2011, Pages 4191–4201, https://doi.org/10.1093/jxb/err122
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026