RT Conference Proceedings
T1 Training memory without aversion: Appetitive hole-board spatial learning increases adult hippocampal neurogenesis.
A1 Sampedro-Piquero, Patricia
A1 Ladrón de Guevara-Miranda, David
A1 Mañas-Padilla, María del Carmen
A1 Gil-Rodríguez, Sara
A1 Gavito, Ana L.
A1 Cano-Pavón, José Manuel
A1 Pedraza-Benítez, María del Carmen
A1 García-Fernández, María
A1 Ladrón de Guevara-Miranda, David
A1 Santín-Núñez, Luis Javier
A1 Castilla-Ortega, María Estela
K1 Neuroplasticidad
AB Learning experiences are potent modulators of adult hippocampalneurogenesis (AHN). However, the vast majority of findings on thelearning-induced regulation of AHN derive from aversively-motivatedtasks, mainly the water maze paradigm, in which stress is a confoundingfactor that affects the AHN outcome. Currently, little is known regardingthe effect of appetitively-motivated training on AHN. Hence we studiedhow spatial learning to find food rewards in a hole-board maze modulatesAHN (cell proliferation and immature neurons) and AHN-related hippocampalneuroplasticity markers (BDNF, IGF-II and CREB phosphorylation) in mice.The 'Trained' mice were tested for both spatial reference and workingmemory and compared to 'Pseudotrained' mice (exposed to different baitedholes in each session, thus avoiding the reference memory component ofthe task) and 'Control' mice (exposed to the maze without rewards). Incontrast to Pseudotrained and Control mice, Trained mice reduced thenumber of proliferating hippocampal cells but they notably increasedtheir population of immature neurons assessed by immunohistochemistry.This evidence shows that hole-board spatial reference learning diminishescell proliferation in favor of enhancing young neurons' survival.Interestingly, the enhanced AHN in the Trained mice (specifically in thesuprapyramidal blade) positively correlated with their reference memoryperformance, but not with their working memory. Furthermore, the Trainedanimals increased the hippocampal protein expression of all theneuroplasticity markers analyzed by western blot. Results show that theappetitively-motivated hole-board task is an useful paradigm topotentiate and/or investigate AHN and hippocampal plasticity minimizingaversive variables such as fear or stress.
YR 2018
FD 2018-07-20
LK https://hdl.handle.net/10630/16326
UL https://hdl.handle.net/10630/16326
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.This study was funded by grants from the Spanish Ministry of Economy andCompetitiveness (Agencia Estatal de Investigación) co-funded by the EuropeanResearch Development Fund -AEI/FEDER, UE- (PSI2015-73156-JIN ‘JóvenesInvestigadores grant’ to E.C.O. and PSI2013-44901-P to L.J.S. and C.P.), from ‘Juntade Andalucía’ SEJ1863 to C.P. and from University of Málaga (Plan Propio 2017 –‘Ayudas para proyectos puente’) to M.G.F.Author P.S.P. holds a ‘Juan de la Cierva-formación‘grant from the Spanish Ministry ofEconomy, Industry and Competitiveness (code: FJCI-2015-23925) and a ‘D.3. Estanciade investigadores de reconocido prestigio en la UMA‘ grant from the University ofMálaga. Authors R.D.M.F. and D.L.G.M. hold ‘FPU’ grants from the Spanish Ministry ofEducation, Culture and Sports (code: FPU14-01610 and FPU13/04819, respectively).Author F.J.P. holds a ‘Miguel Servet’ grant (code: CP14/00212) from the NationalSystem of Health-Instituto de Salud Carlos-III co-funded by FEDER, UE.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 22 ene 2026