RT Journal Article
T1 MiR-2 family regulates insect metamorphosis by controlling the juvenile hormone signaling pathway.
A1 Fernández-Lozano, Jesús
A1 Montañez, Raúl
A1 Bellés, Xavier
K1 Insectos - Metamorfosis
K1 MicroARNs
AB In 2009 we reported that depletion of Dicer-1, the enzyme that catalyzes the final step of miRNA biosynthesis, prevents metamorphosis in Blattella germanica. However, the precise regulatory roles of miRNAs in the process have remained elusive. In the present work, we have observed that Dicer-1 depletion results in an increase of mRNA levels of Krüppel homolog 1 (Kr-h1), a juvenile hormone-dependent transcription factor that represses metamorphosis, and that depletion of Kr-h1 expression in Dicer-1 knockdown individuals rescues metamorphosis. We have also found that the 3′UTR of Kr-h1 mRNA contains a functional binding site for miR-2 family miRNAs (for miR-2, miR-13a, and miR-13b). These data suggest that metamorphosis impairment caused by Dicer-1 and miRNA depletion is due to a deregulation of Kr-h1 expression and that this deregulation is derived from a deficiency of miR-2 miRNAs. We corroborated this by treating the last nymphal instar of B. germanica with an miR-2 inhibitor, which impaired metamorphosis, and by treating Dicer-1-depleted individuals with an miR-2 mimic to allow nymphal-to-adult metamorphosis to proceed. Taken together, the data indicate that miR-2 miRNAs scavenge Kr-h1 transcripts when the transition from nymph to adult should be taking place, thus crucially contributing to the correct culmination of metamorphosis.
PB National Academy of Sciences
YR 2015
FD 2015
LK https://hdl.handle.net/10630/32271
UL https://hdl.handle.net/10630/32271
LA eng
NO Lozano J, Montañez R, Belles X. MiR-2 family regulates insect metamorphosis by controlling the juvenile hormone signaling pathway. Proc Natl Acad Sci U S A. 2015 Mar 24;112(12):3740-5. doi: 10.1073/pnas.1418522112. Epub 2015 Mar 9. PMID: 25775510; PMCID: PMC4378413.
NO Política de acceso abierto tomada de: https://v2.sherpa.ac.uk/id/publication/10338 y https://www.pnas.org/author-center/publication-charges#:~:text=All%20PNAS%20articles%20are%20free,PubMed%20Central%20for%20the%20authors.
NO Government of Catalonia    Ministry of Economy, Industry and Competitiveness    Spanish National Research Council
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 12 abr 2026