RT Conference Proceedings T1 Layered in Time: Exploring the Impact of Nearly 8 Months of Stratification on Carbon Dynamics in a Mediterranean Reservoir. A1 Rodríguez-Gómez, Sofía A1 Montes-Pérez, Jorge A1 Ruiz Nieto, Miriam A1 Ledesma-Hernández, Guillermo A1 Warren-Jiménez, Paula A1 Conejo-Orosa, Teresa A1 Escot, Carmelo A1 Moreno-Ostos, Enrique K1 Ecosistemas mediterráneos K1 Salud de los ecosistemas K1 Carbono - Almacenamiento geológico AB Ecosystem metabolism determines its role as a CO2 source or sink. In lakes and reservoirs,metabolism is conditioned by the phytoplankton community. This relationship was studiedthroughout a hydrological year in El Gergal, a Mediterranean mesotrophic and warmmonomictic reservoir used for water supply to the city of Seville, Spain. Aerobic metabolicrates (respiration and primary production) were modeled using high-resolution data, includingtemperature, PAR, oxygen, salinity, and meteorological variables. The size structure of thephytoplankton community was examined using flow cytometry. Thermal stratificationpersisted for 7.7 months. During this period the aerobic ecosystem metabolism wasautotrophic, with a net O2 production of 0.06 ± 0.03 mg l–1 day–1, and ultraphytoplankton (anintermediate size group between pico- and nanophytoplankton) dominated the community.Notably, the epilimnion was the only layer exhibiting net O2 production, while themetalimnion remained almost neutral and the hypolimnion was heterotrophic. During themixing period, El Gergal was heterotrophic (net O2 consumption: 0.12 ± 0.03 mg l–1 day–1)with picophytoplankton (the smallest size group) dominating the community. On an annualscale, El Gergal reservoir was slightly heterotrophic, raising concerns about its role as a CO2sink YR 2024 FD 2024 LK https://hdl.handle.net/10630/32088 UL https://hdl.handle.net/10630/32088 LA eng DS RIUMA. Repositorio Institucional de la Universidad de Málaga RD 28 feb 2026