RT Journal Article
T1 Characterisation of the mgo operon in Pseudomonas syringae pv. syringae UMAF0158 that is required for mangotoxin production.
A1 Arrebola-Díez, Eva María
A1 Carrión Bravo, Víctor José
A1 Cazorla-López, Francisco Manuel
A1 Pérez-García, Alejandro
A1 Murillo, Jesús
A1 De-Vicente-Moreno, Antonio
K1 Pseudomonas syringae
K1 Bacterias fitopatógenas
K1 Antimetabolitos
K1 Fitotoxinas
AB Background: Mangotoxin is an antimetabolite toxin that is produced by strains of Pseudomonas syringae pv.syringae; mangotoxin-producing strains are primarily isolated from mango tissues with symptoms of bacterial apicalnecrosis. The toxin is an oligopeptide that inhibits ornithine N-acetyl transferase (OAT), a key enzyme in thebiosynthetic pathway of the essential amino acids ornithine and arginine. The involvement of a putativenonribosomal peptide synthetase gene (mgoA) in mangotoxin production and virulence has been reported.Results: In the present study, we performed a RT-PCR analysis, insertional inactivation mutagenesis, a promoterexpression analysis and terminator localisation to study the gene cluster containing the mgoA gene. Additionally, weevaluated the importance of mgoC, mgoA and mgoD in mangotoxin production. A sequence analysis revealed anoperon-like organisation. A promoter sequence was located upstream of the mgoB gene and was found to drive lacZtranscription. Two terminators were located downstream of the mgoD gene. RT-PCR experiments indicated that thefour genes (mgoBCAD) constitute a transcriptional unit. This operon is similar in genetic organisation to those in thethree other P. syringae pathovars for which complete genomes are available (P. syringae pv. syringae B728a, P. syringaepv. tomato DC3000 and P. syringae pv. phaseolicola 1448A). Interestingly, none of these three reference strains is capableof producing mangotoxin. Additionally, extract complementation resulted in a recovery of mangotoxin productionwhen the defective mutant was complemented with wild-type extracts.Conclusions: The results of this study confirm that mgoB, mgoC, mgoA and mgoD function as a transcriptionalunit and operon. While this operon is composed of four genes, only the last three are directly involved inmangotoxin production.
PB BMC
YR 2012
FD 2012-01-17
LK https://hdl.handle.net/10630/30973
UL https://hdl.handle.net/10630/30973
LA eng
NO Arrebola E, Carrión VJ, Cazorla FM, Pérez-García A, Murillo J, de Vicente A. Characterisation of the mgo operon in Pseudomonas syringae pv. syringae UMAF0158 that is required for mangotoxin production. BMC Microbiol. 2012. 12:10.
NO This study was supported by funding from Consejería de Innovación, Cienciay Empresa, Secretaría General de Universidades, Investigación y Tecnología,Junta de Andalucía, Spain (Proyecto de Excelencia P07-AGR-2471),cofinanced by FEDER funds (EU).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026