RT Journal Article
T1 Fruit yield and quality of strawberry plants transformed with a fruit specific strawberry pectate lyase gene.
A1 Youssef, Sabry M.
A1 Jiménez-Bermúdez, Silvia
A1 Bellido, María Luz
A1 Martín-Pizarro, Carmen
A1 Barcelo-Muñoz, Marta
A1 Abdal-Aziz, Samia
A1 Caballero, José Luis
A1 López Aranda, José Manuel
A1 Pliego-Alfaro, Fernando
A1 Muñoz, Juan
A1 Quesada-Felice, Miguel Ángel
A1 Mercado, José A.
K1 Fresas - Cultivo
K1 Genetica vegetal
AB Two transgenic strawberry lines (Pel 1 and Pel 3) containing the open reading frame of a fruit specific strawberry pectate lyase gene (FaplC) under the control of the CaMV35S promoter have been obtained to evaluate the role of this gene on fruit softening. Ripen fruits from both lines showed a significant downregulation of FaplC, being the percentage of silencing of 84 and 71% on Pel 1 and Pel 3, respectively. The agronomic behaviour of transgenic plants was evaluated during two consecutive years. Fruit set increased in the two transgenic lines when compared with control plants, although Pel 1 showed a significant reduction on fruit weight. Firmness of full ripen fruits from Pel lines was significantly higher than control fruits, while color and soluble solids were not affected. The increase of firmness in Pel lines was maintained when ripe fruits were stored for 3 days at 25 8C. Histological analysis of ripe fruits showed lower intercellular spaces and a higher degree of cell to cell contact area in transgenic fruits when compared with controls. Altogether, these results suggest a direct relationship between pectate lyase gene expression and strawberry fruit softening.
PB Elsevier
YR 2009
FD 2009-01-06
LK https://hdl.handle.net/10630/37277
UL https://hdl.handle.net/10630/37277
LA eng
NO Sabry M. Youssef, Silvia Jiménez-Bermúdez, M. Luz Bellido, Carmen Martín-Pizarro, Marta Barceló, Samia Abdallah Abdal-Aziz, José L. Caballero, José M. López-Aranda, Fernando Pliego-Alfaro, Juan Muñoz, Miguel A. Quesada, José A. Mercado, Fruit yield and quality of strawberry plants transformed with a fruit specific strawberry pectate lyase gene, Scientia Horticulturae, Volume 119, Issue 2, 2009, Pages 120-125, ISSN 0304-4238, https://doi.org/10.1016/j.scienta.2008.07.011
NO https://openpolicyfinder.jisc.ac.uk/id/publication/16563
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026