RT Journal Article
T1 A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization.
A1 Sánchez-Vallet, Andrea
A1 Tian, Hui
A1 Rodríguez-Moreno, Luis Gabriel
A1 Valkenburg, Dirk-Jan
A1 Saleem-Batcha, Raspudin
A1 Wawra, Stephan
A1 Kombrink, Anja
A1 Verhage, Leonie
A1 De Jonge, Ronnie
A1 Van Esse, Peter
A1 Zuccaro, Alga
A1 Croll, Daniel
A1 Mesters, Jeroen
A1 Thomma, Bart
K1 Inmunología vegetal
K1 Quitina
K1 Hongos patógenos
AB Plants trigger immune responses upon recognition of fungal cell wall chitin, followed by the release of various antimicrobials, including chitinase enzymes that hydrolyze chitin. In turn, many fungal pathogens secrete LysM effectors that prevent chitin recognition by the host through scavenging of chitin oligomers. We previously showed that intrachain LysM dimerization of the Cladosporium fulvum effector Ecp6 confers an ultrahigh-affinity binding groove that competitively sequesters chitin oligomers from host immune receptors. Additionally, particular LysM effectors are found to protect fungal hyphae against chitinase hydrolysis during host colonization. However, the molecular basis for the protection of fungal cell walls against hydrolysis remained unclear. Here, we determined a crystal structure of the single LysM domain-containing effector Mg1LysM of the wheat pathogen Zymoseptoria tritici and reveal that Mg1LysM is involved in the formation of two kinds of dimers; a chitin-dependent dimer as well as a chitin-independent homodimer. In this manner, Mg1LysM gains the capacity to form a supramolecular structure by chitin-induced oligomerization of chitin-independent Mg1LysM homodimers, a property that confers protection to fungal cell walls against host chitinases. © 2020 Sánchez-Vallet et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PB Public Library of Science
SN 15537366
YR 2020
FD 2020-06-23
LK https://hdl.handle.net/10630/29871
UL https://hdl.handle.net/10630/29871
LA eng
NO Sánchez-Vallet, A., Tian, H., Rodriguez-Moreno, L., Valkenburg, D.-J., Saleem-Batcha, R., Wawra, S., Kombrink, A., Verhage, L., de Jonge, R., van Esse, H. P., Zuccaro, A., Croll, D., Mesters, J. R., & Thomma, B. P. H. J. (2020). A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization. PLOS Pathogens, 16(6), e1008652.
NO The authors acknowledge support from the partners of the European Research Area Network for Coordinating Action in Plant Sciences (ERA-CAPS) consortium “SIPIS”.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026