RT Journal Article
T1 Toxicity of Photobacterium damselae subsp. damselae strains isolated from new cultured marine fish
A1 Labella Vera, Alejandro Manuel
A1 Sanchez-Montes, Nuria
A1 Berbel, Concepción
A1 Aparicio, Manuel
A1 Castro, Dolores
A1 Manchado, Manuel
A1 Borrego, Juan José
K1 Peces - Enfermedades
AB The in vivo and in vitro toxicity of bacterial cells and their extracellular products (ECPs) from 16 strains of Photobacterium damselae subsp. damselae isolated from 7 epizootic outbreaks were evaluated. On the basis of their 50% lethal dose (LD50) values (about 1 × 105 CFU), these strains may be considered as moderately virulent. However, their ECPs were strongly lethal for redbanded seabream Pagrus auriga causing fish death within 2 h post-inoculation (protein concentration ranged between 2.1 and 6.41 μg g–1 fish). The bacterial ECPs tested exhibited several enzymatic activities, such as amylase, lipase, phospholipase, alkaline phosphatase, esterase-lipase, acid phosphatase, and β-glucosaminidase. These ECPs displayed a strong cytotoxic effect on 4 fish and 2 mammalian cell lines, although this activity disappeared when ECPs were heated at 100°C. The virulence of the strains tested could not be related to the hemolytic activity or to the production of the toxindamselysin. Therefore, another unknown type of toxin could play an important role in the virulence mechanisms of this bacterial pathogen.
PB Inter-Research
YR 2010
FD 2010-10-25
LK https://hdl.handle.net/10630/34015
UL https://hdl.handle.net/10630/34015
LA eng
NO Labella A, Sanchez-Montes N, Berbel C, Aparicio M, Castro D, Manchado M, Borrego JJ (2010) Toxicity of Photobacterium damselae subsp. damselae strains isolated from new cultured marine fish. Dis Aquat Org 92:31-40. https://doi.org/10.3354/dao02275
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026